Polymerization-based signal amplification for paper-based immunoassays
Name
Badu-Tawiah-2015-Polymerization-based signal.pdf
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2.1 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Badu-Tawiah, Abraham K.
Lathwal, Shefali
Kaastrup, Kaja
Al-Sayah, Mohammad
Christodouleas, Dionysios C.
Smith, Barbara S.
Whitesides, George M.
Sikes, Hadley
Date Issued
November 2014
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry
Citation
Badu-Tawiah, Abraham K., Shefali Lathwal, Kaja Kaastrup, Mohammad Al-Sayah, Dionysios C. Christodouleas, Barbara S. Smith, George M. Whitesides, and Hadley D. Sikes. “Polymerization-Based Signal Amplification for Paper-Based Immunoassays.” Lab Chip 15, no. 3 (2015): 655–659. © 2015 Royal Society of Chemistry
Version
Final published version
Abstract
Diagnostic tests in resource-limited settings require technologies that are affordable and easy to use with minimal infrastructure. Colorimetric detection methods that produce results that are readable by eye, without reliance on specialized and expensive equipment, have great utility in these settings. We report a colorimetric method that integrates a paper-based immunoassay with a rapid, visible-light-induced polymerization to provide high visual contrast between a positive and a negative result. Using Plasmodium falciparum histidine-rich protein 2 as an example, we demonstrate that this method allows visual detection of proteins in complex matrices such as human serum and provides quantitative information regarding analyte levels when combined with cellphone-based imaging. It also allows the user to decouple the capture of analyte from signal amplification and visualization steps.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
http://dx.doi.org/10.1039/c4lc01239a
