Fast detection of liver fibrosis with collagen-binding single-nanometer iron oxide nanoparticles via T1-weighted MRI
Name
zhang-et-al-2023-fast-detection-of-liver-fibrosis-with-collagen-binding-single-nanometer-iron-oxide-nanoparticles-via.pdf
Description
Published version
Size
1.49 MB
Format
Adobe PDF
Checksum (MD5)
e33eed2bd8d8533b14785d5362d199b3
Author(s)
Zhang, Juanye
Ning, Yingying
Zhu, Hua
Rotile, Nicholas J
Wei, He
Diyabalanage, Himashinie
Hansen, Eric C
Zhou, Iris Y
Barrett, Stephen C
Sojoodi, Mozhdeh
Date Issued
April 24, 2023
Journal
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences of the United States of America
Citation
J. Zhang,Y. Ning,H. Zhu,N.J. Rotile,H. Wei,H. Diyabalanage,E.C. Hansen,I.Y. Zhou,S.C. Barrett,M. Sojoodi,K.K. Tanabe,V. Humblet,A. Jasanoff,P. Caravan, & M.G. Bawendi, Fast detection of liver fibrosis with collagen-binding single-nanometer iron oxide nanoparticles via T1-weighted MRI, Proc. Natl. Acad. Sci. U.S.A. 120 (18) e2220036120.
Version
Final published version
Abstract
SNIO–CBP, a single-nanometer iron oxide (SNIO) nanoparticle functionalized with a type I collagen-binding peptide (CBP), was developed as a T1-weighted MRI contrast agent with only endogenous elements for fast and noninvasive detection of liver fibrosis. SNIO–CBP exhibits 6.7-fold higher relaxivity compared to a molecular gadolinium-based collagen-binding contrast agent CM-101 on a per CBP basis at 4.7 T. Unlike most iron oxide nanoparticles, SNIO–CBP exhibits fast elimination from the bloodstream with a 5.7 min half-life, high renal clearance, and low, transient liver enhancement in healthy mice. We show that a dose of SNIO–CBP that is 2.5-fold lower than that for CM-101 has comparable imaging efficacy in rapid (within 15 min following intravenous injection) detection of hepatotoxin-induced liver fibrosis using T1-weighted MRI in a carbon tetrachloride–induced mouse liver injury model. We further demonstrate the applicability of SNIO–CBP in detecting liver fibrosis in choline-deficient L-amino acid-defined high-fat diet mouse model of nonalcoholic steatohepatitis. These results provide a platform with potential for the development of high relaxivity, gadolinium-free molecular MRI probes for characterizing chronic liver disease.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Biological Engineering
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Creative Commons Attribution-NonCommercial-NoDerivatives
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DOI of Published Version
10.1073/pnas.2220036120
