Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
Name
Sharei-2015-Ex Vivo Cytosolic De.pdf
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1.37 MB
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Checksum (MD5)
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Author(s)
Trifonova, Radiana
Jhunjhunwala, Siddharth
Hartoularos, George C.
Eyerman, Alexandra T.
Lytton-Jean, Abigail K. R.
Angin, Mathieu
Sharma, Siddhartha
Poceviciute, Roberta
Mao, Shirley
Heimann, Megan
Date Issued
April 2015
Journal
PLOS ONE
Publisher
Public Library of Science
Citation
Sharei, Armon, Radiana Trifonova, Siddharth Jhunjhunwala, George C. Hartoularos, Alexandra T. Eyerman, Abigail Lytton-Jean, Mathieu Angin, et al. “Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells.” Edited by Philip A Stumbles. PLOS ONE 10, no. 4 (April 13, 2015): e0118803.
Version
Final published version
Abstract
Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Ragon Institute of MGH, MIT and Harvard
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0118803
