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dc.contributor.advisorPeter T. C. So.en_US
dc.contributor.authorXue, Yi, S.M. Massachusetts Institute of Technologyen_US
dc.contributor.otherMassachusetts Institute of Technology. Department of Mechanical Engineering.en_US
dc.date.accessioned2015-12-16T16:33:25Z
dc.date.available2015-12-16T16:33:25Z
dc.date.copyright2015en_US
dc.date.issued2015en_US
dc.identifier.urihttp://hdl.handle.net/1721.1/100346
dc.descriptionThesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2015.en_US
dc.descriptionCataloged from PDF version of thesis.en_US
dc.descriptionIncludes bibliographical references (pages 65-68).en_US
dc.description.abstractHigh resolution imaging in three dimension is important for biological research. The RESOLFT (Reversible Saturable Optical Fluorescence Transitions) fluorescent microscopy is one technique which can achieve lateral super-resolution imaging. Two-photon microscopy naturally generate high resolution in the longitudinal direction with less background compared to single photon excitation. We combine these two methods to realize three-dimensional high-resolution imaging. This super-resolution method also is limited in imaging speed. We use a spatial light modulator (SLM) as a flexible phase mask of the microscopy. It is used to compensate the system aberration, as well as increasing the imaging speed. The parallel scanning generates multiple super-resolution focuses as an array or in arbitrary positions by phase retrieval calculation. This microscopy combined with SLM control could applied to high throughput 3D imaging or multiple spots tracking in high-resolution.en_US
dc.description.statementofresponsibilityby Yi Xue.en_US
dc.format.extent68 pagesen_US
dc.language.isoengen_US
dc.publisherMassachusetts Institute of Technologyen_US
dc.rightsM.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.en_US
dc.rights.urihttp://dspace.mit.edu/handle/1721.1/7582en_US
dc.subjectMechanical Engineering.en_US
dc.titleParallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator controlen_US
dc.typeThesisen_US
dc.description.degreeS.M.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineering
dc.identifier.oclc931070512en_US


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