Portable iso-dielectrophoresis system
Author(s)
Liu, Lisa, M. Eng. Massachusetts Institute of Technology
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Alternative title
Portable IDS system
Other Contributors
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science.
Advisor
Joel Voldman.
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Metadata
Show full item recordAbstract
Iso-dielectrophoresis (IDS) can be used to characterize and separate cells by their electrical characteristics. An IDS system usually consist of a RF frequency voltage source up to 24 V, syringe pumps, microscope, and computer. Much of this equipment is difficult to transport, making off-site sample collection of time-sensitive samples, such as blood, difficult. The miniaturization of the IDS system would allow measurements to take place at the time of sample collection, improving the confidence in the measurement. This thesis details an attempt to miniaturize each system. The Raspberry Pi microcomputer was used to control and process signals from the hardware. A portable, affordable system for the electrical and optical equipment systems are described. A custom PCB was designed to provide voltage signals up to 3 MHz and 12 Vpp. A microscope using a Raspberry Pi camera and reversed webcam lens achieved 240x magnification with 13 [mu]m resolution. Alternatives to the syringe pump, including air pressure regulated pumps and peristaltic pumps, were considered for the fluidic component.
Description
Thesis: M. Eng., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2016. This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections. Cataloged from student-submitted PDF version of thesis. Includes bibliographical references (pages 60-62).
Date issued
2016Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer SciencePublisher
Massachusetts Institute of Technology
Keywords
Electrical Engineering and Computer Science.