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dc.contributor.authorWurtzel, Omri
dc.contributor.authorPoirier, Amber
dc.contributor.authorSatija, Rahul
dc.contributor.authorCote, Lauren Esther
dc.contributor.authorRegev, Aviv
dc.contributor.authorReddien, Peter
dc.date.accessioned2017-03-27T19:08:40Z
dc.date.available2017-03-27T19:08:40Z
dc.date.issued2015-12
dc.date.submitted2015-10
dc.identifier.issn15345807
dc.identifier.urihttp://hdl.handle.net/1721.1/107733
dc.description.abstractRegeneration starts with injury. Yet how injuries affect gene expression in different cell types and how distinct injuries differ in gene expression remain unclear. We defined the transcriptomes of major cell types of planarians—flatworms that regenerate from nearly any injury—and identified 1,214 tissue-specific markers across 13 cell types. RNA sequencing on 619 single cells revealed that wound-induced genes were expressed either in nearly all cell types or specifically in one of three cell types (stem cells, muscle, or epidermis). Time course experiments following different injuries indicated that a generic wound response is activated with any injury regardless of the regenerative outcome. Only one gene, notum, was differentially expressed early between anterior- and posterior-facing wounds. Injury-specific transcriptional responses emerged 30 hr after injury, involving context-dependent patterning and stem-cell-specialization genes. The regenerative requirement of every injury is different; however, our work demonstrates that all injuries start with a common transcriptional response.en_US
dc.description.sponsorshipBroad Institute of MIT and Harvard. Klarman Cell Observatoryen_US
dc.description.sponsorshipHoward Hughes Medical Institute (Investigator)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH grant R01GM080639)en_US
dc.description.sponsorshipEuropean Molecular Biology Organization (Fellowship)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH grant F32 HD075541)en_US
dc.language.isoen_US
dc.publisherElsevier B.V.en_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.devcel.2015.11.004en_US
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.sourceElsevieren_US
dc.titleA Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planariansen_US
dc.typeArticleen_US
dc.identifier.citationWurtzel, Omri, Lauren E. Cote, Amber Poirier, Rahul Satija, Aviv Regev, and Peter W. Reddien. “A Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planarians.” Developmental Cell 35, no. 5 (December 2015): 632–645.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.mitauthorCote, Lauren Esther
dc.contributor.mitauthorRegev, Aviv
dc.contributor.mitauthorReddien, Peter
dc.relation.journalDevelopmental Cellen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsWurtzel, Omri; Cote, Lauren E.; Poirier, Amber; Satija, Rahul; Regev, Aviv; Reddien, Peter W.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-1772-7447
dc.identifier.orcidhttps://orcid.org/0000-0001-8567-2049
dc.identifier.orcidhttps://orcid.org/0000-0002-5569-333X
mit.licensePUBLISHER_CCen_US


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