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dc.contributor.authorWang, Jigang
dc.contributor.authorZhang, Jianbin
dc.contributor.authorZhang, Chong-Jing
dc.contributor.authorWong, Yin Kwan
dc.contributor.authorLim, Teck Kwang
dc.contributor.authorHua, Zi-Chun
dc.contributor.authorLiu, Bin
dc.contributor.authorShen, Han-Ming
dc.contributor.authorLin, Qingsong
dc.contributor.authorTannenbaum, Steven R
dc.date.accessioned2017-04-21T22:57:16Z
dc.date.available2017-04-21T22:57:16Z
dc.date.issued2016-02
dc.date.submitted2015-10
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/1721.1/108372
dc.description.abstractTo date, the exact targets and mechanism of action of curcumin, a natural product with anti-inflammatory and anti-cancer properties, remain elusive. Here we synthesized a cell permeable curcumin probe (Cur-P) with an alkyne moiety, which can be tagged with biotin for affinity enrichment, or with a fluorescent dye for visualization of the direct-binding protein targets of curcumin in situ. iTRAQ™ quantitative proteomics approach was applied to distinguish the specific binding targets from the non-specific ones. In total, 197 proteins were confidently identified as curcumin binding targets from HCT116 colon cancer cell line. Gene Ontology analysis showed that the targets are broadly distributed and enriched in the nucleus, mitochondria and plasma membrane, and they are involved in various biological functions including metabolic process, regulation, response to stimulus and cellular process. Ingenuity Pathway Analysis™ (IPA) suggested that curcumin may exert its anticancer effects over multiple critical biological pathways including the EIF2, eIF4/p70S6K, mTOR signaling and mitochondrial dysfunction pathways. Functional validations confirmed that curcumin downregulates cellular protein synthesis, and induces autophagy, lysosomal activation and increased ROS production, thus leading to cell death.en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/srep22146en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceNature Publishing Groupen_US
dc.titleIn situ Proteomic Profiling of Curcumin Targets in HCT116 Colon Cancer Cell Lineen_US
dc.typeArticleen_US
dc.identifier.citationWang, Jigang, Jianbin Zhang, Chong-Jing Zhang, Yin Kwan Wong, Teck Kwang Lim, Zi-Chun Hua, Bin Liu, Steven R. Tannenbaum, Han-Ming Shen, and Qingsong Lin. “In Situ Proteomic Profiling of Curcumin Targets in HCT116 Colon Cancer Cell Line.” Scientific Reports 6 (February 26, 2016): 22146. © 2016 Macmillan Publishers Limiteden_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistryen_US
dc.contributor.mitauthorTannenbaum, Steven R
dc.relation.journalScientific Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsWang, Jigang; Zhang, Jianbin; Zhang, Chong-Jing; Wong, Yin Kwan; Lim, Teck Kwang; Hua, Zi-Chun; Liu, Bin; Tannenbaum, Steven R.; Shen, Han-Ming; Lin, Qingsongen_US
dspace.embargo.termsNen_US
mit.licenseOPEN_ACCESS_POLICYen_US


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