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Generating single-domain antibodies against fibronectin splice variants

Author(s)
Peterson, Alexis W
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Massachusetts Institute of Technology. Department of Biology.
Advisor
Richard Hynes.
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MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582
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Abstract
Here, I describe the process of generating single-domain antibodies which bind to splice variants of fibronectin containing EIIIA or EIIIB. Alpacas were immunized with either purified antigen cocktails, or from the ECM of tumor samples, and antibody libraries were generated. Using these libraries to pan against, I selected for VHH which bind to EIIIA and EIIIB. Since these splice variants are upregulated in tumor angiogenesis and are rarely seen elsewhere in adult tissues, antibodies targeting EIIIA or EIIIB may be of use for imaging tumors and metastases.
Description
Thesis: S.M., Massachusetts Institute of Technology, Department of Biology, 2017.
 
Cataloged from PDF version of thesis.
 
Includes bibliographical references (page 51).
 
Date issued
2017
URI
http://hdl.handle.net/1721.1/108893
Department
Massachusetts Institute of Technology. Department of Biology
Publisher
Massachusetts Institute of Technology
Keywords
Biology.

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