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dc.contributor.authorRowlands, Christopher
dc.contributor.authorPark, Jong Kang
dc.contributor.authorSo, Peter T. C.
dc.date.accessioned2017-06-22T14:49:18Z
dc.date.available2017-06-22T14:49:18Z
dc.date.issued2017-03
dc.date.submitted2017-02
dc.identifier.issn2072-666X
dc.identifier.urihttp://hdl.handle.net/1721.1/110164
dc.description.abstractTemporal focusing multiphoton microscopy is a technique for performing highly parallelized multiphoton microscopy while still maintaining depth discrimination. While the conventional wide-field configuration for temporal focusing suffers from sub-optimal axial resolution, line scanning temporal focusing, implemented here using a digital micromirror device (DMD), can provide substantial improvement. The DMD-based line scanning temporal focusing technique dynamically trades off the degree of parallelization, and hence imaging speed, for axial resolution, allowing performance parameters to be adapted to the experimental requirements. We demonstrate this new instrument in calibration specimens and in biological specimens, including a mouse kidney slice.en_US
dc.description.sponsorshipUnited States. National Institutes of Health (5-P41-EB015871-28)en_US
dc.description.sponsorshipUnited States. National Institutes of Health (2R01EY017656-06)en_US
dc.description.sponsorshipUnited States. National Institutes of Health (R21-NS091982-01)en_US
dc.description.sponsorshipUnited States. National Institutes of Health (1-U01-NS090438-01)en_US
dc.description.sponsorshipUnited States. National Institutes of Health (1U01CA202177-01)en_US
dc.description.sponsorshipUnited States. National Institutes of Health (1R01HL121386-01A1)en_US
dc.language.isoen_US
dc.publisherMDPI AGen_US
dc.relation.isversionofhttp://dx.doi.org/10.3390/mi8030085en_US
dc.rightsCreative Commons Attribution 4.0 International Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceMDPIen_US
dc.titleEnhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Deviceen_US
dc.typeArticleen_US
dc.identifier.citationPark, Jong; Rowlands, Christopher and So, Peter. “Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device.” Micromachines 8, no. 3 (March 2017): 85 © 2017 The Author(s)en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Laser Biomedical Research Centeren_US
dc.contributor.mitauthorPark, Jong Kang
dc.contributor.mitauthorSo, Peter T. C.
dc.relation.journalMicromachinesen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsPark, Jong; Rowlands, Christopher; So, Peteren_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-4698-6488
mit.licensePUBLISHER_CCen_US


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