Clonogenic Neoblasts Are Pluripotent Adult Stem Cells That Underlie Planarian Regeneration
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Reddien_Clonogenic neoblasts.pdf
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Author(s)
Wagner, D. E.
Wang, Irving E.
Reddien, Peter
Date Issued
May 2011
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Wagner, D. E.; Wang, I. E. and Reddien, P. W. “Clonogenic Neoblasts Are Pluripotent Adult Stem Cells That Underlie Planarian Regeneration.” Science 332, 6031 (May 2011): 811–816 © 2011 American Association for the Advancement of Science (AAAS)
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Author's final manuscript
Abstract
Pluripotent cells in the embryo can generate all cell types, but lineage-restricted cells are generally thought to replenish adult tissues. Planarians are flatworms and regenerate from tiny body fragments, a process requiring a population of proliferating cells (neoblasts). Whether regeneration is accomplished by pluripotent cells or by the collective activity of multiple lineage-restricted cell types is unknown. We used ionizing radiation and single-cell transplantation to identify neoblasts that can form large descendant-cell colonies in vivo. These clonogenic neoblasts (cNeoblasts) produce cells that differentiate into neuronal, intestinal, and other known postmitotic cell types and are distributed throughout the body. Single transplanted cNeoblasts restored regeneration in lethally irradiated hosts. We conclude that broadly distributed, adult pluripotent stem cells underlie the remarkable regenerative abilities of planarians.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1126/science.1203983
