In vitro and ex vivo strategies for intracellular delivery
Author(s)Sahay, Gaurav; Stewart, Martin P; Sharei, Armon Reza; Ding, Xiaoyun Sean; Langer, Robert S; Jensen, Klavs F; ... Show more Show less
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Intracellular delivery of materials has become a critical component of genome-editing approaches, ex vivo cell-based therapies, and a diversity of fundamental research applications. Limitations of current technologies motivate development of next-generation systems that can deliver a broad variety of cargo to diverse cell types. Here we review in vitro and ex vivo intracellular delivery approaches with a focus on mechanisms, challenges and opportunities. In particular, we emphasize membrane-disruption-based delivery methods and the transformative role of nanotechnology, microfluidics and laboratory-on-chip technology in advancing the field.
DepartmentDavid H. Koch Institute for Integrative Cancer Research at MIT; Massachusetts Institute of Technology. Department of Chemical Engineering
Nature Publishing Group
Stewart, Martin P.; Sharei, Armon; Ding, Xiaoyun et al. “In Vitro and Ex Vivo Strategies for Intracellular Delivery.” Nature 538, 7624 (October 2016): 183–192 © 2016 Macmillan Publishers Limited, part of Springer Nature
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