dc.contributor.author | Sahay, Gaurav | |
dc.contributor.author | Stewart, Martin P | |
dc.contributor.author | Sharei, Armon Reza | |
dc.contributor.author | Ding, Xiaoyun Sean | |
dc.contributor.author | Langer, Robert S | |
dc.contributor.author | Jensen, Klavs F | |
dc.date.accessioned | 2017-08-04T13:47:16Z | |
dc.date.available | 2017-08-04T13:47:16Z | |
dc.date.issued | 2016-10 | |
dc.date.submitted | 2015-03 | |
dc.identifier.issn | 0028-0836 | |
dc.identifier.issn | 1476-4687 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/110923 | |
dc.description.abstract | Intracellular delivery of materials has become a critical component of genome-editing approaches, ex vivo cell-based therapies, and a diversity of fundamental research applications. Limitations of current technologies motivate development of next-generation systems that can deliver a broad variety of cargo to diverse cell types. Here we review in vitro and ex vivo intracellular delivery approaches with a focus on mechanisms, challenges and opportunities. In particular, we emphasize membrane-disruption-based delivery methods and the transformative role of nanotechnology, microfluidics and laboratory-on-chip technology in advancing the field. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (R01GM101420-01A1) | en_US |
dc.language.iso | en_US | |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/nature19764 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | Prof. Jensen via Erja Kajosalo | en_US |
dc.title | In vitro and ex vivo strategies for intracellular delivery | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Stewart, Martin P.; Sharei, Armon; Ding, Xiaoyun et al. “In Vitro and Ex Vivo Strategies for Intracellular Delivery.” Nature 538, 7624 (October 2016): 183–192 © 2016 Macmillan Publishers Limited, part of Springer Nature | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemical Engineering | en_US |
dc.contributor.department | Koch Institute for Integrative Cancer Research at MIT | en_US |
dc.contributor.approver | Jensen, Klavs F. | en_US |
dc.contributor.mitauthor | Stewart, Martin P | |
dc.contributor.mitauthor | Sharei, Armon Reza | |
dc.contributor.mitauthor | Ding, Xiaoyun Sean | |
dc.contributor.mitauthor | Langer, Robert S | |
dc.contributor.mitauthor | Jensen, Klavs F | |
dc.relation.journal | Nature | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Stewart, Martin P.; Sharei, Armon; Ding, Xiaoyun; Sahay, Gaurav; Langer, Robert; Jensen, Klavs F. | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-4112-6622 | |
dc.identifier.orcid | https://orcid.org/0000-0003-4252-9335 | |
dc.identifier.orcid | https://orcid.org/0000-0003-4255-0492 | |
dc.identifier.orcid | https://orcid.org/0000-0001-7192-580X | |
mit.license | PUBLISHER_POLICY | en_US |