Show simple item record

dc.contributor.authorWang, Wenjing
dc.contributor.authorWildes, Craig P
dc.contributor.authorPattarabanjird, Tanyaporn
dc.contributor.authorSanchez, Mateo I
dc.contributor.authorGlober, Gordon F
dc.contributor.authorMatthews, Gillian A
dc.contributor.authorTye, Kay M
dc.contributor.authorTing, Alice Y
dc.date.accessioned2018-01-12T16:00:07Z
dc.date.available2018-01-12T16:00:07Z
dc.date.issued2017-09
dc.date.submitted2016-11
dc.identifier.issn1087-0156
dc.identifier.issn1546-1696
dc.identifier.urihttp://hdl.handle.net/1721.1/113080
dc.description.abstractActivity remodels neurons, altering their molecular, structural, and electrical characteristics. To enable the selective characterization and manipulation of these neurons, we present FLARE, an engineered transcription factor that drives expression of fluorescent proteins, opsins, and other genetically encoded tools only in the subset of neurons that experienced activity during a user-defined time window. FLARE senses the coincidence of elevated cytosolic calcium and externally applied blue light, which together produce translocation of a membrane-anchored transcription factor to the nucleus to drive expression of any transgene. In cultured rat neurons, FLARE gives a light-to-dark signal ratio of 120 and a high-to low-calcium signal ratio of 10 after 10 min of stimulation. Opsin expression permitted functional manipulation of FLARE-marked neurons. In adult mice, FLARE also gave light- A nd motor-activity-dependent transcription in the cortex. Due to its modular design, minute-scale temporal resolution, and minimal dark-state leak, FLARE should be useful for the study of activity-dependent processes in neurons and other cells that signal with calcium.en_US
dc.description.sponsorshipMassachusetts Institute of Technologyen_US
dc.description.sponsorshipStanford Universityen_US
dc.description.sponsorshipJPB Foundationen_US
dc.description.sponsorshipPicower Institute for Learning and Memory (Engineering Award)en_US
dc.description.sponsorshipNational Institute of Mental Health (U.S.) (R01-MH102441-01)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Director's New Innovator Award DP2-DK-102256-01)en_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nbt.3909en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleA light- and calcium-gated transcription factor for imaging and manipulating activated neuronsen_US
dc.typeArticleen_US
dc.identifier.citationWang, Wenjing, et al. “A Light- and Calcium-Gated Transcription Factor for Imaging and Manipulating Activated Neurons.” Nature Biotechnology, vol. 35, no. 9, June 2017, pp. 864–71.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistryen_US
dc.contributor.mitauthorWang, Wenjing
dc.contributor.mitauthorPattarabanjird, Tanyaporn
dc.contributor.mitauthorTing, Alice Y
dc.relation.journalNature Biotechnologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2018-01-11T17:10:02Z
dspace.orderedauthorsWang, Wenjing; Wildes, Craig P; Pattarabanjird, Tanyaporn; Sanchez, Mateo I; Glober, Gordon F; Matthews, Gillian A; Tye, Kay M; Ting, Alice Yen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-1967-2889
dc.identifier.orcidhttps://orcid.org/0000-0002-8277-5226
mit.licenseOPEN_ACCESS_POLICYen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record