Show simple item record

dc.contributor.authorWang, Yejun
dc.contributor.authorNagarajan, Mallika
dc.contributor.authorUhler, Caroline
dc.contributor.authorShivashankar, G. V.
dc.date.accessioned2018-03-14T18:31:29Z
dc.date.available2018-03-14T18:31:29Z
dc.date.issued2017-06
dc.date.submitted2017-06
dc.identifier.issn1059-1524
dc.identifier.issn1939-4586
dc.identifier.urihttp://hdl.handle.net/1721.1/114156
dc.description.abstractExtracellular matrix signals from the microenvironment regulate gene expression patterns and cell behavior. Using a combination of experiments and geometric models, we demonstrate correlations between cell geometry, three-dimensional (3D) organization of chromosome territories, and gene expression. Fluorescence in situ hybridization experiments showed that micropatterned fibroblasts cultured on anisotropic versus isotropic substrates resulted in repositioning of specific chromosomes, which contained genes that were differentially regulated by cell geometries. Experiments combined with ellipsoid packing models revealed that the mechanosensitivity of chromosomes was correlated with their orientation in the nucleus. Transcription inhibition experiments suggested that the intermingling degree was more sensitive to global changes in transcription than to chromosome radial positioning and its orientations. These results suggested that cell geometry modulated 3D chromosome arrangement, and their neighborhoods correlated with gene expression patterns in a predictable manner. This is central to understanding geometric control of genetic programs involved in cellular homeostasis and the associated diseases.en_US
dc.description.sponsorshipAustrian Science Fund (Y 903-N35)en_US
dc.description.sponsorshipMechanobiology Institute, Singaporeen_US
dc.description.sponsorshipSingapore. Ministry of Education (Academic Research Fund Tier3 MOE2012-T3-1-001)en_US
dc.publisherAmerican Society for Cell Biology (ASCB)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1091/MBC.E16-12-0825en_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourceAmerican Society for Cell Biologyen_US
dc.titleOrientation and repositioning of chromosomes correlate with cell geometry–dependent gene expressionen_US
dc.typeArticleen_US
dc.identifier.citationWang, Yejun, et al. “Orientation and Repositioning of Chromosomes Correlate with Cell Geometry–dependent Gene Expression.” Molecular Biology of the Cell, edited by Alex Dunn, vol. 28, no. 14, July 2017, pp. 1997–2009.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.mitauthorUhler, Caroline
dc.relation.journalMolecular Biology of the Cellen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2018-02-23T19:31:38Z
dspace.orderedauthorsWang, Yejun; Nagarajan, Mallika; Uhler, Caroline; Shivashankar, G. V.en_US
dspace.embargo.termsNen_US
mit.licensePUBLISHER_CCen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record