High-throughput multiplexed transcriptome and protein measurements in single cells

Author(s)
Shivaei, Shirin
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Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science.
Advisor
Fei Chen.
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MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582
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Abstract
We present cell gels, a platform for high-throughput multiplexed measurements of transcriptome and proteome in single cells. This method takes advantage of droplet barcoding and hydrogel chemistry to capture mRNAs and proteins in thousands of cells. We report the challenges of acquiring sequencing data from cell gels due to complex mechanisms underlying bead based library prep in polyacrylamide gels. We show the applications of this method in detecting intracellular proteins, sorting based on intracellular markers after cell lysis, and expanding thousands of cells in single droplets.
Description
Thesis: M. Eng., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2018.
 
This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
 
Cataloged from student-submitted PDF version of thesis.
 
Includes bibliographical references (pages 45-47).
 
Date issued
2018
URI
http://hdl.handle.net/1721.1/119713
Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Publisher
Massachusetts Institute of Technology
Keywords
Electrical Engineering and Computer Science.
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