Show simple item record

dc.contributor.authorMichaelson, Jarett
dc.contributor.authorChoi, Heejin
dc.contributor.authorSo, Peter T. C.
dc.contributor.authorHuang, Hayden
dc.date.accessioned2019-02-12T16:30:29Z
dc.date.available2019-02-12T16:30:29Z
dc.date.issued2012-06
dc.identifier.isbn978-0-7918-4480-9
dc.identifier.urihttp://hdl.handle.net/1721.1/120342
dc.description.abstractImmortalized cells are commonly used as analogs for primary cells in many cell mechanics, tissue engineering, and biochemical assays. However, it is not well-established whether immortal cell lines can mimic the behavior of primary cells in more physiological (three-dimensional) environments. For this project, we investigate the mechanical properties of primary cardiac fibroblasts (CFs) and 3T3 transformed fibroblasts when cultured in cell bi-layers by comparing the cellsʼ viscoelastic properties.Many cellular and tissue processes depends critically on the viscoelastic properties of the cell (1,2). Additionally, changes in a cellʼs mechanical properties are correlated with some disease states (3). Particle tracking microrheology (PTM) is an ideal technique for measuring the passive, internal viscoelastic properties of cells in more physiological constructs, since it does not require direct contact with the cells. We found that the top layer of 3T3 cells in the bi-layer was significantly stiffer than the bottom layer, while CFs did not exhibit any significant difference between layers. These data suggest that 3T3 cells may exhibit different cytoskeletal behavior in 3D culturing scenarios compared to primary fibroblasts.en_US
dc.publisherASME Internationalen_US
dc.relation.isversionofhttp://dx.doi.org/10.1115/SBC2012-80385en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceASMEen_US
dc.titleMechanical Properties of Primary and Immortal Fibroblasts in Cell Bi-Layersen_US
dc.typeArticleen_US
dc.identifier.citationMichaelson, Jarett, Heejin Choi, Peter So, and Hayden Huang. “Mechanical Properties of Primary and Immortal Fibroblasts in Cell Bi-Layers.” ASME 2012 Summer Bioengineering Conference, Parts A and B (June 20, 2012).en_US
dc.contributor.departmentInstitute for Medical Engineering and Scienceen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.mitauthorChoi, Heejin
dc.contributor.mitauthorSo, Peter T. C.
dc.contributor.mitauthorHuang, Hayden
dc.relation.journalASME 2012 Summer Bioengineering Conference, Parts A and Ben_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/ConferencePaperen_US
eprint.statushttp://purl.org/eprint/status/NonPeerRevieweden_US
dc.date.updated2019-01-03T17:58:03Z
dspace.orderedauthorsMichaelson, Jarett; Choi, Heejin; So, Peter; Huang, Haydenen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-3681-7410
dc.identifier.orcidhttps://orcid.org/0000-0003-4698-6488
mit.licensePUBLISHER_POLICYen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record