Developing VHH-based tools to study Ebolavirus infection
Author(s)Nguyen, Jason V. M. H.(Jason Vu Minh Hien)
Developing Visible Human Viewer-based tools to study Ebolavirus infection
Massachusetts Institute of Technology. Department of Biology.
Hidde L. Ploegh.
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Variable domains of camelid-derived heavy chain-only antibodies, or VHHs, have emerged as a unique antigen binding moiety that holds promise in its versatility and utilization as a tool to study biological questions. This thesis focuses on two aspects on developing tools to study infectious disease, specifically Ebolavirus entry. In Chapter 1, I provide an overview about antibodies and how antibodies have transformed the biomedical field and how single domain antibody fragments, or VHHs, have entered this arena. I will also touch upon how VHHs have been used in various fields and certain aspects that remain underexplored. Chapter 2 focuses on the utilization of VHHs to study Ebolavirus entry using VHHs that were isolated from alpacas. Two VHHs were found to neutralize Ebolavirus in both Biosafety Level 2 and 4 laboratory conditions. Ongoing experiments to address mechanism focuses on two aspects of neutralization: Cathepsin inhibition or NPC1-mediated inhibition. Finally, Chapter 3 discusses the overall landscape for Ebolavirus therapeutics and will discuss future directions of this work.
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2019Cataloged from PDF version of thesis.Includes bibliographical references.
DepartmentMassachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology