Measuring light transport properties using speckle patterns as structured illumination

Abstract

The measurement of light absorption and scattering properties of biological materials has several diagnostic and therapeutic applications. We can measure these properties for skin without contact using structured illumination and imaging. However, building simple handheld devices remains challenging due to motion artefacts and moving targets. To overcome this limitation, we project random speckle patterns instead of discrete spatial frequencies on the target. Since random patterns are spatially broadband, they capture more information per image, enabling frame-by-frame analysis. In this paper, we describe the statistics of objective speckles and demonstrate how the optical system is designed for spatially bandlimited illumination. Next, we use a diverse set of liquid tissue phantom to validate the method. We successfully demonstrate that a calibrated instrument can independently predict the two primary light transport properties of a homogeneous turbid system. This work is a starting point for analysing skin and other heterogeneous biological media in the future.