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dc.contributor.authorJohnson, Grace E.
dc.contributor.authorLi, Gene-Wei
dc.date.accessioned2020-05-12T12:51:33Z
dc.date.available2020-05-12T12:51:33Z
dc.date.issued2018-12
dc.identifier.issn1557-7988
dc.identifier.urihttps://hdl.handle.net/1721.1/125169
dc.description.abstractBacteria produce different amounts of their proteins in response to different conditions. The ability to accurately quantitate the rates of protein synthesis across the genome is an important step toward understanding both underlying regulation and bacterial physiology at a systems level. Ribosome profiling (deep sequencing of ribosome-protected mRNA fragments) enables accurate and high-throughput measurement of such synthesis rates. Ribosomes protect RNAs from nuclease digestion; thus, by collecting and sequencing protected footprints, one can obtain information on the position of every ribosome at the time of cell collection. Assuming ribosomes go on to translate full-length proteins, the density of ribosomes across an ORF can be used to determine protein synthesis rates. Here we outline a step-by-step protocol and discuss the steps where variability and bias may be introduced, including ways to minimize it.en_US
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/bs.mie.2018.08.031en_US
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.sourcePMCen_US
dc.titleGenome-Wide Quantitation of Protein Synthesis Rates in Bacteriaen_US
dc.typeArticleen_US
dc.identifier.citationJohnson, Grace E. and Gene-Wei Li. “Genome-Wide Quantitation of Protein Synthesis Rates in Bacteria.” Methods in enzymology 612 (2018): 225-249 © 2018 The Author(s)en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.relation.journalMethods in enzymologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2020-01-23T17:36:38Z
dspace.date.submission2020-01-23T17:36:41Z
mit.journal.volume612en_US


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