Show simple item record

dc.contributor.authorAbraham, Eytan
dc.contributor.authorGadish, Or
dc.contributor.authorFranses, Joseph W.
dc.contributor.authorChitalia, Vipul C.
dc.contributor.authorArtzi, Natalie
dc.contributor.authorEdelman, Elazer R
dc.date.accessioned2020-07-22T14:45:47Z
dc.date.available2020-07-22T14:45:47Z
dc.date.issued2017-07
dc.date.submitted2017-05
dc.identifier.issn2366-7478
dc.identifier.urihttps://hdl.handle.net/1721.1/126307
dc.description.abstractCulture of endothelial cells (ECs) embedded in 3D scaffolds of denatured collagen has shown tremendous therapeutic potential in clinical trials of tissue repair. It is postulated that these matrix-embedded ECs (MEECs) attain a differential phenotype similar to early progenitor forms, which cannot be attained in 2D culture. MEECs are compared to 2D-ECs and endothelial progenitor cells (EPCs) by secretome, phenotype, and genetic fingerprint, and are found to be altered from 2D-ECs on all levels, adopting an EPC-like phenotype. This manifests in elevation of CD34 expression—a progenitor cell marker—and protein secretion and gene expression profiles that are similar to EPCs. Even more striking is that EPCs in 2D lose their phenotype, evident by the loss of CD34 expression, but are able to regain expression over time when embedded in the same 3D matrices, suggesting that future in vitro EPC work should use ME-EPCs to recapitulate in vivo phenotype. These findings elucidate the relationship between EPCs and the substratum-dependent regulation imparted by ECs which is critical to understand in order to optimize MEEC therapy and propel it into the clinic.en_US
dc.description.sponsorshipNational Institutes of Health (Grant R01-GM49039)en_US
dc.language.isoen
dc.publisherWileyen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/adbi.201700057en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleMatrix-Embedded Endothelial Cells Attain a Progenitor-Like Phenotypeen_US
dc.typeArticleen_US
dc.identifier.citationAbraham, Eytan et al. "Matrix‐Embedded Endothelial Cells Attain a Progenitor‐Like Phenotype." Advanced BioSystems 1, 9 (July 2017): 1700057 © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheimen_US
dc.contributor.departmentMassachusetts Institute of Technology. Institute for Medical Engineering and Scienceen_US
dc.relation.journalAdvanced BioSystemsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-10-09T17:23:33Z
dspace.date.submission2019-10-09T17:23:37Z
mit.journal.volume1en_US
mit.journal.issue9en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record