Macrolide and fluoroquinolone associated mutations in Mycoplasma genitalium in a retrospective study of male and female patients seeking care at a STI Clinic in Guangzhou, China, 2016-2018
Name
12879_2020_Article_5659.pdf
Size
509.09 KB
Format
Adobe PDF
Checksum (MD5)
4372fd787d2361c1a7b80bb8f97fdb95
Author(s)
Tso, Lai Sze
Date Issued
December 11, 2020
Journal
BMC infectious diseases
Publisher
BioMed Central
Citation
Ke, Wujian et al. “Macrolide and fluoroquinolone associated mutations in Mycoplasma genitalium in a retrospective study of male and female patients seeking care at a STI Clinic in Guangzhou, China, 2016-2018.” BMC infectious diseases, 20 (December 2020): 950 © 2020 The Author(s)
Version
Final published version
Abstract
Background:Antimicrobial resistance inM. genitaliumis a growing clinical problem. We investigated the mutations associatedwith macrolide and fluoroquinolone resistance, two commonly used medical regimens for treatment in China. Our aim is toanalyze the prevalence and diversity of mutations amongM. genitalium-positive clinical specimens in Guangzhou, south China.Methods:A total of 154 storedM. genitaliumpositive specimens from men and womenattending a STI clinic were testedfor macrolide and fluoroquinolone mutations.M. genitaliumwas detected via TaqMan MGB real-time PCR. Mutationsassociated with macrolide resistance were detected using primers targeting region V of the 23S rRNA gene.Fluoroquinolone resistant mutations were screened via primers targeting topoisomerase IV (parC)andDNAgyrase(gyrA).Results:98.7% (152/154), 95.5% (147/154) and 90.3% (139/154) ofM. genitaliumpositive samples produced sufficientamplicon for detecting resistance mutations in 23S rRNA,gyrAandparCgenes, respectively. 66.4% (101/152), 0.7% (1/147)and 77.7% (108/139) samples manifested mutations in 23S rRNA,gyrAandparCgenes, respectively. A2072G (59/101,58.4%) and S83I (79/108, 73.1%) were highly predominating in 23S rRNA andparCgenes, respectively. Two samples hadamino acid substitutions ingyrA(M95I and A96T, respectively). Two samples had two amino acid substitutions inparC(S83I + D87Y). 48.6% (67/138) of samples harbored both macrolide and fluoroquinolone resistance-associated mutations.The most common combination of mutations was A2072G (23S rRNA) and S83I (parC) (40/67, 59.7%). One sample hadthree amino acid changes in 23S rRNA,gyrAandparCgenes (A2072G + A96T + S83I). Conclusions:The high antimicrobial resistance rate ofM. genitaliumin Guangzhou is a very worrying problem andsuggests that antimicrobial resistance testing and the development of new antibiotic regimens are crucially needed.
MIT Department
Massachusetts Institute of Technology. Department of Anthropology
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1186/s12879-020-05659-3
