Search
Now showing items 1-10 of 43
Development and characterization of immunogenic genetically engineered mouse models of pancreatic cancer
(Massachusetts Institute of Technology, 2020)
Insights into mechanisms of immune escape have fueled the clinical success of immunotherapy in many cancers. However, pancreatic cancer has remained largely refractory to checkpoint immunotherapy. To uncover mechanisms of ...
The persistence of haploinsufficiency and its role in genome evolution
(Massachusetts Institute of Technology, 2020)
In diploid organisms there are two copies of every gene, one from each parent. While the majority of genes are robust to deletion of one of the two copies, a subset of genes remains highly dosage sensitive, causing a ...
The biochemical basis for the cooperative action of microRNAs
(Massachusetts Institute of Technology, 2020)
In metazoans, microRNAs (miRNAs) act to repress mRNAs through a combination of translational repression and target degradation. miRNAs predominantly pair within the 3' untranslated region (3' UTR) of the mRNA. In cells, ...
Regulators of the Drosophila oocyte-to-embryo transition
(Massachusetts Institute of Technology, 2020)
The transition from oocyte to embryo is critical across metazoans, as it marks the onset of development and is essential for fertility. Although many important regulators of the oocyte-to-embryo transition have been ...
The roles of the helicase double-hexamer complex and the ssDNA-binding protein RPA during eukaryotic DNA replication
(Massachusetts Institute of Technology, 2020)
Eukaryotic DNA replication is a complex process that must occur accurately, completely, and only once per cell cycle. To accomplish these goals, the events of DNA replication are tightly coupled to cell-cycle progression. ...
Comprehensive single-cell transcriptional profiling of the regenerative planarian Schmidtea mediterranea
(Massachusetts Institute of Technology, 2020)
Animals can contain hundreds of cell types, each of which has a distinct morphology and function. The transcriptome of a cell dictates this unique cell biology. Recent approaches for high throughput single-cell RNA sequencing ...
Tool development for the rapid identification of microbiome manipulating agents
(Massachusetts Institute of Technology, 2020)
Manipulation of complex microbial communities, such as human microbiomes, plays a critical role in the study and treatment of microbiome associated diseases. However, the tools available to perform microbiome manipulations ...
Characterizing the ecology of coral reef microorganisms across different scales within the Caribbean
(Massachusetts Institute of Technology, 2020)
Microorganisms sustain the high productivity of coral reefs and support one of the most diverse, valuable, and threatened ecosystems on Earth. Despite the importance of reef microorganisms, there is a lack of understanding ...
The genetic landscape of protein-protein interaction specificity
(Massachusetts Institute of Technology, 2020)
Protein-protein interaction specificity is often encoded at the primary sequence level, and by just a few interfacial residues. Collectively, these residues have both positive and negative roles, promoting a desired, cognate ...
Mapping the therapy resistance landscapes of acute leukemias using in vivo functional genomics
(Massachusetts Institute of Technology, 2020)
The recurrence of therapy resistant disease remains an intractable problem in oncology clinical care. To address this issue, investigators have traditionally focused on elucidating cell-intrinsic mechanisms that render ...