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Structure and dynamics of full-length M2 protein of influenza A virus from solid-state NMR
(Massachusetts Institute of Technology, 2017)
Solid-state nuclear magnetic resonance (SSNMR) has been frequently used to elucidate the structure and dynamics of membrane proteins and fibrils that are difficult to characterize by Xray crystallography or solution NMR. ...
Enzyme engineering by directed evolution and applications in cellular imaging and proteomic mapping
(Massachusetts Institute of Technology, 2016)
A thorough understanding of biological systems on a molecular level requires tools to study individual protein components within the native cellular context. A wide panoply of enzyme-mediated labeling methodologies have ...
Discovery and investigation of the novel overall activity allosteric regulation of the Bacillus subtilis class Ib ribonucleotide reductase
(Massachusetts Institute of Technology, 2017)
Ribonucleotide reductases (RNRs) catalyze the reduction of nucleotides to 2'-deoxynucleotides in all organisms. Class lb RNRs consist of two subunits: a houses the catalytic and allosteric effector binding sites, and p ...
Photoinduced phase transitions and irreversible dynamics studied with single-shot spectroscopy
(Massachusetts Institute of Technology, 2016)
Single-shot femtosecond spectroscopy was developed to study irreversible processes and materials far from equilibrium. It was then applied to investigate photoinduced phase transitions in semimetals and manganites. The ...
High-oxidation-state molybdenum and tungsten monoalkoxide pyrrolide alkylidenes as catalysts for olefin metathesis
(Massachusetts Institute of Technology, 2014)
Chapter 1 describes work toward solid-supported W olefin metathesis catalysts. Attempts to tether derivatives of the known Z-selective catalyst W(NAr)(C₃H₆)(pyr)(OHIPT) (Ar = 2,6- diisopropylphenyl, pyr = pyrrolide; HIPT ...
I. Persistent radicals for dynamic nuclear polarization : II. Synthesis of substituted indoles
(Massachusetts Institute of Technology, 2015)
Part I: An brief introduction to dynamic nuclear polarization (DNP) is provided including a discussion of polarization mechanisms and development of new polarization methods in a historical context. Efficient synthesis of ...
Palladium reagents for bioconjugation
(Massachusetts Institute of Technology, 2018)
physicochemical properties in comparison to their linear counterparts. Here we detail a method for a divergent macrocyclization of unprotected peptides by crosslinking two cysteine residues with bis-palladium organometallic ...
Protective antigen-mediated delivery of biomolecules
(Massachusetts Institute of Technology, 2018)
The intracellular delivery of therapeutic biomolecules such as oligonucleotides and proteins remains a key challenge today. Protective antigen, a naturally evolved protein translocase derived from Bacillus anthracis, has ...
Liberation of low-coordinate phosphorus species from anthracene-based molecular precursors
(Massachusetts Institute of Technology, 2018)
The study of heavier p-block analogs to unsaturated organic small molecules is of fundamental importance, allowing insight into the differing bonding and reactivity patterns that control their synthetic utility. A major ...
Engineering LuxR-type quorum sensing proteins for new functions
(Massachusetts Institute of Technology, 2019)
Bacteria communicate information in a process known as quorum sensing, actuating downstream gene expression based on cell-cell signalling. Cell-cell signalling allows for complex and multi-cellular behavior otherwise ...