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mRNA stability in soma and neurites of cultured neuronal cells
(Massachusetts Institute of Technology, 2017)
Neurons rely on mRNA localization and local protein synthesis in neurites to regulate synaptic plasticity, axonogenesis and neural signaling. The local regulation of mRNA stability in neurites is poorly understood. To ...
Generating single-domain antibodies against fibronectin splice variants
(Massachusetts Institute of Technology, 2017)
Here, I describe the process of generating single-domain antibodies which bind to splice variants of fibronectin containing EIIIA or EIIIB. Alpacas were immunized with either purified antigen cocktails, or from the ECM of ...
Effects of cell growth and a mobile genetic element on propagation of the phages SP16 and SP-beta in Bacillus subtilis
(Massachusetts Institute of Technology, 2014)
Two studies were performed on Bacillus subtilis phages SP16 and SP-beta to characterize the effects of growth and an integrative conjugative element, ICEBs1, respectively. I found that B. subtilis strains are significantly ...
Analysis of NusA's roles in DNA repair and damage tolerance pathways in Escherichia coli
(Massachusetts Institute of Technology, 2015)
NusA has long known to be a bona fide transcription factor that functions in both termination and antitermination. However, its role in DNA repair and damage tolerance has not been explored until recently. Cohen et al. ...
A role for the Speedy gene family in the early stages of mammalian meiosis
(Massachusetts Institute of Technology, 2008)
Meiosis is the process by which a diploid cell undergoes two sequential rounds of division without an intervening round of DNA replication. The result is the formation of haploid gametes. The genes and signals that regulate ...
Use of bgaH as a reporter gene for studying translation initiation in the archaeon Haloferax volcanii
(Massachusetts Institute of Technology, 2008)
The bgaH gene isolated from Haloferax lucentensis codes for P-galactosidase. To study the function of initiator tRNAs in translation initiation in Haloferax volcanii, the initiator AUG codon of the bgaH gene was mutated ...
Localization and function of the Drosophila huntingtin protein
(Massachusetts Institute of Technology, 2007)
Huntington's Disease (HD) is an autosomal dominant neurodegenerative disorder caused by an expansion of a polyglutamine tract in the huntingtin protein. This mutation leads to conformational instability, resulting in ...
Physiological IRE-1-XBP-1 and PEK-1 signaling in Caenorhabditis elegans larval development and immunity
(Massachusetts Institute of Technology, 2012)
The endoplasmic reticulum (ER) is responsible for the folding and processing of approximately one third of proteins in eukaryotic cells, and homeostasis in this compartment is tightly regulated. The Unfolded Protein Response, ...
Interpreting the genome physically in-silico
(Massachusetts Institute of Technology, 2017)
The relation between heavy metal effluents and benthic infaunal community changes in a coastal embayment in Maine.
(Massachusetts Institute of Technology, 1973)