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Biological detection by means of mass reduction in a suspended microchannel resonator
(Massachusetts Institute of Technology, 2004)
Label-free detection is the detection of biomolecules and their interactions, without the use of a molecule external to the interaction, used as a reporter to indicate presence and/or location. The suspended microchannel ...
High-throughput genomic phenotyping
(Massachusetts Institute of Technology, 2004)
In the wake of the development of technology to sequence the complete genome of an organism, it has become expedient to generate methodologies to elucidate and characterize the function of all genes constituting the complete ...
Investigation of growth factors and cytokines that suppress adult stem cell asymmetric cell kinetics
(Massachusetts Institute of Technology, 2005)
Adult stem cells are potentially useful in many biomedical applications that can save lives and increase the quality of a patient's life, such as tissue engineering, cell replacement, and gene therapy. However, these ...
Using optical tweezers, single molecule fluorescence and the ZIF268 protein-DNA system to probe mechanotransduction mechanisms
(Massachusetts Institute of Technology, 2006)
Optical tweezers instruments use laser radiation pressure to trap microscopic dielectric beads. With the appropriate chemistry, such a bead can be attached to a single molecule as a handle, permitting the application of ...
DNA alkylation repair deficient mice are susceptible to chemically induced Inflammatory Bowel Disease
(Massachusetts Institute of Technology, 2006)
The two most common forms of inflammatory bowel disease (IBD) are ulcerative colitis (UC) and Crohn's Disease (CD), which affect more than 1 million Americans. Recently the incidence of IBD has been rising in Japan, Europe ...
Quantitative analysis of TLR-4-mediated cell responses in murine macrophages
(Massachusetts Institute of Technology, 2008)
TLR-4 is essential in host defense against bacterial infection. By recognition of specific pathogen-associated molecular patterns such as lipopolysaccharide (LPS), TLR-4 can in tandem initiate a pair of downstream signaling ...
Investigating late stage biopharmaceutical product loss using novel analytical and process technology
(Massachusetts Institute of Technology, 2008)
The biopharmaceutical industry uses recombinant protein technologies to provide novel therapeutics to patients around the world. These technologies have presented exciting opportunities for breakthrough medical treatments ...
A novel high-throughput in-cell Western assay for the quantitative measurement of signaling dynamics in DNA damage signaling networks : cell decision processes in response to DNA double strand breaks
(Massachusetts Institute of Technology, 2006)
Following exposure to DNA damage, cells initiate a stress response involving multiple protein kinase signaling cascades. The DNA damage response results in one of several possible cell-fate decisions, or cellular responses: ...
The detection of immortal DNA strand co-segregation as a method of adult stem cell identification
(Massachusetts Institute of Technology, 2004)
The study of stem cells is one of the most fascinating topics in biology. Adult stem cells (ASC), which play the prime role in the maintenance and restoration of tissues, are thought to hold great potential for the advancement ...
Transcriptional response of O⁶-methylguanine methyltransferase deficient yeast to methyl-N-nitro-N-nitrosoguanidine (MNNG)
(Massachusetts Institute of Technology, 2004)
(cont.) of transcription factors and subsequently, induction of RNA processing (35% of genes incrementally induced) and kinases involved in protein phosphorylation. In the WT, the response was restricted to a transient ...