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Biodegradable microfluidic scaffolds for vascular tissue engineering
(Massachusetts Institute of Technology, 2004)
This work describes the integration of novel microfabrication techniques for vascular tissue engineering applications in the context of a novel biodegradable elastomer. The field of tissue engineering and organ regeneration ...
A central composite design to investigate antibody fragment production by Pichia pastoris
(Massachusetts Institute of Technology, 2005)
This study aims to investigate the relationships between growth parameters (agitation, glycerol concentration, salt concentration) and responses (biomass, growth rate, protein expression), by a 3-factor-3-level central ...
Biological detection by means of mass reduction in a suspended microchannel resonator
(Massachusetts Institute of Technology, 2004)
Label-free detection is the detection of biomolecules and their interactions, without the use of a molecule external to the interaction, used as a reporter to indicate presence and/or location. The suspended microchannel ...
High-throughput genomic phenotyping
(Massachusetts Institute of Technology, 2004)
In the wake of the development of technology to sequence the complete genome of an organism, it has become expedient to generate methodologies to elucidate and characterize the function of all genes constituting the complete ...
Murine embryonic stem cells and hypoxia : growth kinetics, metabolism, and plating efficiency
(Massachusetts Institute of Technology, 2004)
There is reason to believe that embryonic stem cells would grow favorably in a hypoxic environment. These cells come from the pre-implantation embryo, whose native environment is the hypoxic mammalian reproductive tract. ...
Fundamental differentiation and growth characterization of murine embryonic stem cells in varied culture conditions
(Massachusetts Institute of Technology, 2004)
Although embryonic stem (ES) cells and their pluripotent capability have been elucidated for decades, little study has been done on obtaining the pluripotency profile of ES cells in the incipient stages of differentiation. ...
Investigation of growth factors and cytokines that suppress adult stem cell asymmetric cell kinetics
(Massachusetts Institute of Technology, 2005)
Adult stem cells are potentially useful in many biomedical applications that can save lives and increase the quality of a patient's life, such as tissue engineering, cell replacement, and gene therapy. However, these ...
Using optical tweezers, single molecule fluorescence and the ZIF268 protein-DNA system to probe mechanotransduction mechanisms
(Massachusetts Institute of Technology, 2006)
Optical tweezers instruments use laser radiation pressure to trap microscopic dielectric beads. With the appropriate chemistry, such a bead can be attached to a single molecule as a handle, permitting the application of ...
The carcinogenicity, mutagenicity, and toxicity of arsenic and cadmium
(Massachusetts Institute of Technology, 2004)
Recent epidemiological data has indicated that chronic exposure to metals such as arsenic and cadmium increases the risk of cancer and other diseases. These metals may have negative biological effects on cells by disrupting ...
Molecular mechanism of interactions between estrogen receptor and estrogen receptor selective genotoxins
(Massachusetts Institute of Technology, 2000)
Although one million new breast cancer cases arise each year worldwide, therapies to treat the disease are limited. Conventional treatments including the chemotherapeutic agent, Tamoxifen, have had only limited success, ...