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dc.contributor.authorCorbridge, Sarah M.
dc.contributor.authorRice, Richard C.
dc.contributor.authorBean, Linda A.
dc.contributor.authorWüthrich, Christian
dc.contributor.authorDang, Xin
dc.contributor.authorNicholson, Daniel A.
dc.contributor.authorKoralnik, Igor J.
dc.date.accessioned2021-09-16T18:37:49Z
dc.date.available2021-09-16T18:37:49Z
dc.date.issued2019-04
dc.date.submitted2018-12
dc.identifier.issn1355-0284
dc.identifier.issn1538-2443
dc.identifier.urihttps://hdl.handle.net/1721.1/131279
dc.description.abstractJC virus (JCV) can cause a lytic infection of oligodendrocytes and astrocytes in the central nervous system (CNS) leading to progressive multifocal leukoencephalopathy (PML). JCV can also infect meningeal and choroid plexus cells causing JCV meningitis (JCVM). Whether JCV also infects meningeal and choroid plexus cells in PML patients and other immunosuppressed individuals with no overt symptoms of meningitis remains unknown. We therefore analyzed archival formalin-fixed, paraffin-embedded brain samples from PML patients, and HIV-seropositive and seronegative control subjects by immunohistochemistry for the presence of JCV early regulatory T Ag and JCV VP1 late capsid protein. In meninges, we detected JCV T Ag in 11/48 (22.9%) and JCV VP1 protein in 8/48 (16.7%) PML patients. In choroid plexi, we detected JCV T Ag in 1/7 (14.2%) and JCV VP1 protein in 1/8 (12.5%) PML patients. Neither JCV T Ag nor VP1 protein could be detected in meninges or choroid plexus of HIV-seropositive and HIV-seronegative control subjects without PML. In addition, examination of underlying cerebellar cortex of PML patients revealed JCV-infected cells in the molecular layer, including GAD 67+ interneurons, but not in HIV-seropositive and HIV-seronegative control subjects without PML. Our findings suggest that productive JCV infection of meningeal cells and choroid plexus cells also occurs in PML patients without signs or symptoms of meningitis. The phenotypic characterization of JCV-infected neurons in the molecular layer deserves further study. This data provides new insight into JCV pathogenesis in the CNS.en_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.isversionofhttps://doi.org/10.1007/s13365-019-00753-yen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceSpringer International Publishingen_US
dc.titleJC virus infection of meningeal and choroid plexus cells in patients with progressive multifocal leukoencephalopathyen_US
dc.typeArticleen_US
dc.identifier.citationCorbridge, Sarah M. et al. "JC virus infection of meningeal and choroid plexus cells in patients with progressive multifocal leukoencephalopathy." Journal of NeuroVirology 25, 4 (April 2019): 520–524. © 2019 Journal of NeuroVirology, Incen_US
dc.contributor.departmentMcGovern Institute for Brain Research at MITen_US
dc.relation.journalJournal of NeuroVirologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2020-09-24T21:16:17Z
dc.language.rfc3066en
dc.rights.holderJournal of NeuroVirology, Inc.
dspace.embargo.termsY
dspace.date.submission2020-09-24T21:16:17Z
mit.journal.volume25en_US
mit.journal.issue4en_US
mit.licensePUBLISHER_POLICY
mit.metadata.statusCompleteen_US
mit.metadata.statusAuthority Work and Publication Information Needed


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