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dc.contributor.authorAvello, Monika
dc.contributor.authorDavis, Kathleen P
dc.contributor.authorGrossman, Alan Davis
dc.date.accessioned2022-07-21T13:27:25Z
dc.date.available2021-09-20T18:22:09Z
dc.date.available2022-07-21T13:27:25Z
dc.date.issued2019
dc.identifier.urihttps://hdl.handle.net/1721.1/132387.2
dc.description.abstract© 2019 John Wiley & Sons Ltd Integrative and conjugative elements (ICEs) are mobile genetic elements that transfer from cell to cell by conjugation (like plasmids) and integrate into the chromosomes of bacterial hosts (like lysogenic phages or transposons). ICEs are prevalent in bacterial chromosomes and play a major role in bacterial evolution by promoting horizontal gene transfer. Exclusion prevents the redundant transfer of conjugative elements into host cells that already contain a copy of the element. Exclusion has been characterized mostly for conjugative elements of Gram-negative bacteria. Here, we report the identification and characterization of an exclusion mechanism in ICEBs1 from the Gram-positive bacterium Bacillus subtilis. We found that cells containing ICEBs1 inhibit the activity of the ICEBs1-encoded conjugation machinery in other cells. This inhibition (exclusion) was specific to the cognate conjugation machinery and the ICEBs1 gene yddJ was both necessary and sufficient to mediate exclusion by recipient cells. Through a mutagenesis and enrichment screen, we identified exclusion-resistant mutations in the ICEBs1 gene conG. Using genes from a heterologous but related ICE, we found that the exclusion specificity was determined by ConG and YddJ. Finally, we found that under conditions that support conjugation, exclusion provides a selective advantage to the element and its host cells.en_US
dc.language.isoen
dc.publisherWileyen_US
dc.relation.isversionof10.1111/MMI.14359en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleIdentification, characterization and benefits of an exclusion system in an integrative and conjugative element of Bacillus subtilisen_US
dc.typeArticleen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.relation.journalMolecular Microbiologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2020-10-19T14:20:13Z
dspace.orderedauthorsAvello, M; Davis, KP; Grossman, ADen_US
dspace.date.submission2020-10-19T14:20:17Z
mit.journal.volume112en_US
mit.journal.issue4en_US
mit.licenseOPEN_ACCESS_POLICY
mit.metadata.statusPublication Information Neededen_US


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