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dc.contributor.authorBrandão, Hugo B.
dc.contributor.authorGabriele, Michele
dc.contributor.authorHansen, Anders S
dc.date.accessioned2021-12-13T16:42:01Z
dc.date.available2021-10-27T19:51:49Z
dc.date.available2021-12-13T16:03:53Z
dc.date.available2021-12-13T16:42:01Z
dc.date.issued2020-12
dc.identifier.issn0955-0674
dc.identifier.urihttps://hdl.handle.net/1721.1/133256.3
dc.description.abstract© 2020 The Author(s) Mammalian genomes are organized and regulated through long-range chromatin interactions. Structural loops formed by CCCTC-binding factor (CTCF) and cohesin fold the genome into domains, while enhancers interact with promoters across vast genomic distances to regulate gene expression. Although genomics and fixed-cell imaging approaches help illuminate many aspects of chromatin interactions, temporal information is usually lost. Here, we discuss how 3D super-resolution live-cell imaging (SRLCI) can resolve open questions on the dynamic formation and dissolution of chromatin interactions. We discuss SRLCI experimental design, implementation strategies, and data interpretation and highlight associated pitfalls. We conclude that, while technically demanding, SRLCI approaches will likely emerge as a critical tool to dynamically probe 3D genome structure and function and to study enhancer–promoter interactions and chromatin looping.en_US
dc.description.sponsorshipNational Institutes of Health (Grants R00GM130896, DP2GM140938)en_US
dc.language.isoen
dc.publisherElsevier BVen_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/J.CEB.2020.11.002en_US
dc.rightsCreative Commons Attribution 4.0 International licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourceElsevieren_US
dc.titleTracking and interpreting long-range chromatin interactions with super-resolution live-cell imagingen_US
dc.typeArticleen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.relation.journalCurrent Opinion in Cell Biologyen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2021-08-27T18:31:15Z
dspace.orderedauthorsBrandão, HB; Gabriele, M; Hansen, ASen_US
dspace.date.submission2021-08-27T18:31:17Z
mit.journal.volume70en_US
mit.licensePUBLISHER_CC
mit.metadata.statusPublication Information Neededen_US


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