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dc.contributor.authorZanotto, Gustavo
dc.contributor.authorLiebesny, Paul
dc.contributor.authorBarrett, Myra
dc.contributor.authorZlotnick, Hannah
dc.contributor.authorGrodzinsky, Alan
dc.contributor.authorFrisbie, David
dc.date.accessioned2021-10-27T20:35:10Z
dc.date.available2021-10-27T20:35:10Z
dc.date.issued2019
dc.identifier.urihttps://hdl.handle.net/1721.1/136393
dc.description.abstract© 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. The objective of this study was to improve cartilage repair and integration using self-assembling KLD hydrogel functionalized with platelet-derived growth factor-BB and heparin-binding insulin-like growth factor-1 with associated enzymatic trypsin pre-treatment of the native cartilage. Bilateral osteochondral defects were created at the central portion of the femoral trochlear groove of 48 skeletally mature, white New Zealand rabbits. One limb received a randomly assigned treatment and the contralateral limb served as the control. Treated defects were exposed to trypsin for 2 min and filled with self-assembling KLD hydrogel only, or associated to growth factors. All control limbs received KLD hydrogel alone or received only trypsin but not hydrogel. Ninety days post-defect creation, the rabbits were euthanized and magnetic resonance imaging, radiography, macroscopic evaluation, histology, and immunohistochemistry of the joint and repaired tissue were performed. Mixed model analyses of variance were utilized to assess the outcome parameters and individual comparisons were performed using Least Square Means procedure and differences with p-value ' 0.05 were considered significant. Trypsin enzymatic pre-treatment improved cellular morphology, cluster formation and subchondral bone reconstitution. Platelet-derived growth factor-BB improved subchondral bone healing and basal integration. Heparin-binding insulin-like growth factor-1 associated with platelet-derived growth factor improved tissue and cell morphology. The authors conclude that self-assembling KLD hydrogel functionalized with platelet-derived growth factor and heparin-binding insulin-like growth factor-1 with associated enzymatic pre-treatment of the native cartilage with trypsin resulted in an improvement on the cartilage repair process. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2307–2315, 2019.
dc.language.isoen
dc.publisherWiley
dc.relation.isversionof10.1002/JOR.24414
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourcePMC
dc.titleTrypsin Pre‐Treatment Combined With Growth Factor Functionalized Self‐Assembling Peptide Hydrogel Improves Cartilage Repair in Rabbit Model
dc.typeArticle
dc.relation.journalJournal of Orthopaedic Research
dc.eprint.versionAuthor's final manuscript
dc.type.urihttp://purl.org/eprint/type/JournalArticle
eprint.statushttp://purl.org/eprint/status/PeerReviewed
dc.date.updated2020-11-19T19:55:28Z
dspace.orderedauthorsZanotto, G; Liebesny, P; Barrett, M; Zlotnick, H; Grodzinsky, A; Frisbie, D
dspace.date.submission2020-11-19T19:55:31Z
mit.journal.volume37
mit.journal.issue11
mit.licenseOPEN_ACCESS_POLICY
mit.metadata.statusAuthority Work and Publication Information Needed


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