Engineering thermostable affinity proteins for use in high-throughput immunoassay formats

Author(s)
Jia, Huan; Moomen, Nazirulmubin Abdul; Leong, Jeanette; Kongsuphol, Patthara; Lim, Zhi Feng Sherman; ... Show more
Thumbnail
DownloadPublished version (848.8Kb)
Publisher with Creative Commons License
Terms of use
Creative Commons Attribution-Noncommercial http://creativecommons.org/licenses/by-nc/3.0/
Metadata
Show full item record
Abstract
ELISA utilizes polystyrene materials for capture reagents. Natural absorption randomly orients the capture reagent, decreasing immobilization and target biomarker accessibility. A thermostable affinity protein rcSso7d can replace antibodies as a capture reagent in cellulose-based diagnostic tests. ‘RAFIASRRIRRP’ at the N-terminus of two repeating units of rcSso7d improved polystyrene immobilization for high-throughput ELISA.
Date issued
2023
URI
https://hdl.handle.net/1721.1/156917
Department
Singapore-MIT Alliance in Research and Technology (SMART); Massachusetts Institute of Technology. Department of Chemical Engineering
Journal
Sensors & Diagnostics
Publisher
Royal Society of Chemistry
Citation
Sens. Diagn., 2023,2, 627-631
Version: Final published version
Collections