Engineering Disease Resistance in a Reservoir Species for the Mice Against Ticks Project

Abstract

This thesis explores the application of genome editing technologies to combat zoonotic infectious diseases through the development of a novel heritable immunization strategy targeting reservoir species. Focusing on Lyme disease, where white-footed mice (Peromyscus leucopus) serve as the primary reservoir, we propose embedding immunity into the germline of these animals to disrupt the disease transmission cycle and reduce the prevalence of the disease in the environment. By establishing genome engineering protocols for Peromyscus and demonstrating heritable protection against Lyme disease in genetically engineered Mus musculus, we show the feasibility of heritable immunization for long-term disease prevention. This work highlights the potential of genetic engineering for ecological interventions, offering a novel approach to public health challenges while fostering responsible community engagement in ecosystem engineering.