Browsing Synthetic Biology by Title

Now showing items 80-99 of 139

    • Idempotent Vector Design for Standard Assembly of Biobricks 

      Knight, Thomas (MIT Artificial Intelligence Laboratory; MIT Synthetic Biology Working Group, 2003)
      The lack of standardization in assembly techniques for DNA sequences forces each DNA assembly reaction to be both an experimental tool for addressing the current research topic, and an experiment in and of itself. One ...

    • iGEM 2005 Invitation Supplement 

      Endy, Drew (2005-09-29)
      This document provides background material in support of the 2005 intercollegiate Genetically Engineered Machine Competition (aka iGEM). Herein, you can read quick summaries of earlier competitions and courses, obtain a ...

    • Impact of Synthetic Genomics on the Threat of Bioterrorism with Viral Agents 

      Collett, Marc (2007-12-04)
      In 2002, a team of researchers at the State University of New York led by Eckard Wimmer assembled a DNA template for the RNA poliovirus using an internet-available nucleotide sequence and mail order synthetic ...

    • The Imperative of Synthetic Biology: A Proposed National Research Initiative 

      Lazowska, Ed; Endy, Drew (2008-12-23)

    • In the valley of the shadow of death 

      Brent, Roger (2006-11-22)
      During the 20th century, advances in biological understanding sparked a global revolution in biological capability, or RBC. Since that time, the revolution has proceeded in a Moore's-law-like fashion for many decades. ...

    • Instructions to BBF RFC Authors 

      Tarjan, Daniel; Sleight, Sean; Shetty, Reshma; Ridgway, Doug; Rettberg, Randy; e.a. (OpenWetWare, 2008-11-10)
      This BioBricks Foundation Request for Comments (BBF RFC) provides in- formation about the preparation and submission of BBF RFCs to The Bio- Bricks Foundation (BBF).

    • Integrases, Aliens, & Bill Joy 

      Endy, Drew (2013-03-27)
      Research presentation on recent and unpublished work before the MIT Synthetic Biology Working Group on 24 September 2012 in MIT Building 56.

    • LabView VIs for operating Sortostat 

      Kelly, Jason (2008-05-14)

    • Large-Scale Peptide Modification on BioBrick Proteins 

      Tian, Feng; Chen, GuoQiang; Wang, Zhao (2009-10-30)
      With the established BioBrick Assembly standards, fusion proteins with diversified functions are easy to manipulate. However, we realized that the existing BBF RFC do not clearly defining the standard for peptide display ...

    • May 2006 correspondance between James Randerson and Drew Endy 

      Endy, Drew (2006-06-15)
      I am a science journalist with the Guardian newspaper in London. We are a national daily paper. I am hoping to put together an investigative piece on how easy (or not) it would be for a would-be terrorist to put together ...

    • Measurement of bacterial promoter absolute activity and determination of dynamic performance in cell-free expression system by qPCR 

      Olchowik, Anna; Lower, Michal; Moreno, Cherry; Pankowski, Jaroslaw (2010-12-05)
      The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of bacterial promoters. The absolute activity can be experimentally measured in Moles of RNA per Moles of DNA in time, ...

    • The Measurement of rho‐independent Transcription Terminator Efficiency 

      He, Jiankui; Jing, Shui; Zhang, Mengshi; Liu, Xianggeng; Chen, Yao; e.a. (2012-10-11)
      The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of terminators in bacteria. Because we have characterized the performance of terminator in E. coli and used a simple ...

    • miMeasure – a standard for miRNA binding site characterization in mammalian cells 

      Adlung, Lorenz; Sabah, Jude Al; Bayer, Philipp; Berrens, Rebecca; Cristiano, Elena; e.a. (2010-12-05)
      This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, ...

    • The minimum information for a qualified BioBrick 

      Zhou, Mubing; Zhang, Junqiu; He, Jiankui (2012-10-11)
      Since the information of many existing BioBricks is incomplete, thus the usage of the BioBricks will be affected. It is necessary to standardize the minimum information required for a qualified BioBrick. Furthermore this ...

    • miTuner - a kit for microRNA based gene expression tuning in mammalian cells 

      Adlung, Lorenz; Sabah, Jude Al; Bayer, Philipp; Berrens, Rebecca; Cristiano, Elena; e.a. (2010-12-05)
      The purpose of this RFC is to introduce a modular expression tuning kit for use in mammalian cells. The kit enables the regulation of the gene expression of any gene of interest (GOI) based on synthetic microRNAs, endogenous ...

    • MONOD, a Collaborative Tool for Manipulating Biological Knowledge 

      Soergel, David; Choi, Kirindi; Thomson, Ty; Doane, Jay; George, Brian; e.a. (2008-06-19)
      We describe an open source software tool called MONOD, for Modeler’s Notebook and Datastore, designed to capture and communicate knowledge generated during the process of building models of many-component biological ...

    • Naming of standards of physical composition of BioBrick parts 

      Rettberg, Randy; Shetty, Reshma (2009-04-17)
      This BioBricks Foundation Request for Comments (BBF RFC) proposes a naming convention for standards of physical composition of BioBrick parts in which assembly standards are named according to the BBF RFC that specifies ...

    • A New Biobrick Assembly Strategy Designed for Facile Protein Engineering 

      Phillips, Ira; Silver, Pamela (2006-04-20)
      The existing biobrick assembly technique[1] provides a straightforward way to combine standardized biological components, termed biobricks. This system, however, is limited in that each protein-encoding biobrick must ...

    • A New Standard to Connect BioBrick Parts for Precise Extraction of an Enzyme Digestion Product 

      Uekusa, Kousuke; Iguchi, Seiko (2010-12-05)
      This BioBricks Foundation Request for Comments (BBF RFC) introduces a new standard to connect BioBrick parts using BglI site.

    • Novel Normalization Standard using Fluorescence 

      Bukhari, Syed Habib Tahir; Akkineni, Ashwini Rahul; Ananth, Adithya; Gordeev, Victor; Grieb, Svea; e.a. (2010-12-05)
      The Biotec_Dresden Team 2010 developed an approach where two fluorescent proteins are simultaneously expressed. The fact that one reporter, in our case RFP, is constitutively expressed allows to monitor cell growth. Secondly, ...