Cytokine-capture nanoparticles to quantify IL-2 concentrations within the immunological synapse during T cell activation
Author(s)Hong, Julee Y. (Julee Yang-A.), 1980-
Massachusetts Institute of Technology. Dept. of Materials Science and Engineering.
Darrell J. Irvine.
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The feasibility of bringing a nascent technology for detection and quantification of local cytokine concentrations on cell surfaces to market is presented in this paper. Quantum dots or fluorochrome-loaded nanoparticles are conjugated with antibodies for target analytes and with proteins that allow nanoparticle attachment to the surface of T cells. A second labeled monoclonal antibody is introduced to detect the presence of any captured-cytokines using 3D fluorescent microscopy or flow cytometry. Microscopy of DO.11 cells labeled with cytokinecapture particles have shown successful detection of exogenous IL2. A comparison of existing patents with cytokine-capture technology revealed that although each aspect of the device is covered by prior IP, the capabilities of the technology exceed the claimed uses of the individual components. A preliminary market research for cytokine-capture technology applications resulted in dismissing the immunoassay industry as a target market. However, T cell monitoring was identified as a far more lucrative industry.
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2003.Includes bibliographical references (leaf 29).
DepartmentMassachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology
Materials Science and Engineering.