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Design, Fabrication and Functional Analysis of a New Protein Array Based on ssDNA-based Assembly

Author(s)
Ajikumar, Parayil Kumaran; Ng, Jin Kiat; Tang, Yew Chung; Lee, Jim Yang; Stephanopoulos, Gregory; Too, Heng-Phon; ... Show more Show less
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Abstract
In the post genomic era, proteomics has enormous potential in biology and medicine. Among the various bioanalytical tools developed, protein microarray is one of the recent advancements which offer high throughput profiling of cellular proteins to provide insights into the mechanisms of biological processes. Fundamentally, the protein microarray involves the immobilization of interacting elements, proteins, on a few square microns of a solid support and in principle, it is capable of detecting analytes with a higher sensitivity than conventional macroscopic immunoassays. Here in the present report we delineates the design, fabrication and functional analysis of protein microarray using semi-synthetic ssDNA tagged-proteins as capturing moiety as well as address on a solid support. Optimization of the platform has been carried out by investigating various parameters such as surface chemistry, signal amplification, and conditions for homogenous liguid phase protein-protein interaction.
Date issued
2006-01
URI
http://hdl.handle.net/1721.1/30384
Series/Report no.
Molecular Engineering of Biological and Chemical Systems (MEBCS)
Keywords
Proteomics, Protein micro array, Dendrimer, glass slide, ssDNA-antibody

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