A New Biobrick Assembly Strategy Designed for Facile Protein Engineering
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PhillipsSilverFusion.pdf
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Author(s)
Phillips, Ira
Silver, Pamela
Date Issued
April 20, 2006
Abstract
The existing biobrick assembly technique[1] provides a straightforward
way to combine standardized biological components, termed biobricks.
This system, however, is limited in that each protein-encoding biobrick
must contain a complete translated region. Signal sequences or other protein
domains often convey a specific function to the protein to which they
are attached; hence, each domain should be considered an independent
biological part. With the current assembly technique, assembling such
parts is not possible. This paper presents a revised assembly strategy
that is compatible with the current biobrick definition and permits the
construction of fusion proteins.
Subjects
BioBricks
Standard Assembly
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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