A New Biobrick Assembly Strategy Designed for Facile Protein Engineering
Author(s)Phillips, Ira; Silver, Pamela
The existing biobrick assembly technique provides a straightforward way to combine standardized biological components, termed biobricks. This system, however, is limited in that each protein-encoding biobrick must contain a complete translated region. Signal sequences or other protein domains often convey a specific function to the protein to which they are attached; hence, each domain should be considered an independent biological part. With the current assembly technique, assembling such parts is not possible. This paper presents a revised assembly strategy that is compatible with the current biobrick definition and permits the construction of fusion proteins.
BioBricks, Standard Assembly
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