Design and testing of the thermal properties of the structure of an ultra high-throughput mutational spectrometer
Author(s)
Del Zio, Michael R. (Michael Robert), 1982-![Thumbnail](/bitstream/handle/1721.1/32822/57615152-MIT.pdf.jpg?sequence=5&isAllowed=y)
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Other Contributors
Massachusetts Institute of Technology. Dept. of Mechanical Engineering.
Advisor
Ian W. Hunter.
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A process known as mutational spectrometry allows the detection of both single and multiple mutations that appear to be spontaneous, using a technique known as constant denaturing capillary electrophoresis (CDCE). CDCE requires a region of constant temperature and concentration of denaturant. A massively parallel, fully automated instrument, capable of handling as many as 10,000 DNA samples simultaneously, is suited to this technique. A modular structure of such a mutational spectrometer was designed to remain water-tight, provide an array to hold the capillaries for electrophoretic excitation, and modulate the flow of a heat transfer fluid. Six such modules were manufactured and assembled. As the heat transfer fluid passed through the assembled structure, the natural thermal loss was determined.
Description
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004. Includes bibliographical references (leaves 42-43).
Date issued
2004Department
Massachusetts Institute of Technology. Department of Mechanical EngineeringPublisher
Massachusetts Institute of Technology
Keywords
Mechanical Engineering.