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dc.contributor.advisorIan Hunter.en_US
dc.contributor.authorChia, Helena Nien-Hwa, 1982-en_US
dc.contributor.otherMassachusetts Institute of Technology. Dept. of Mechanical Engineering.en_US
dc.date.accessioned2006-05-15T20:33:02Z
dc.date.available2006-05-15T20:33:02Z
dc.date.copyright2004en_US
dc.date.issued2004en_US
dc.identifier.urihttp://hdl.handle.net/1721.1/32828
dc.descriptionThesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004.en_US
dc.descriptionIncludes bibliographical references (leaf 21).en_US
dc.description.abstractTissue print technology allows for the transfer of cellular material from tissue onto a nitrocellulose film for immunocytochemical assays. The MIT BioInstrumentation Laboratory is currently developing a novel cancer marker imaging system for detection of cancerous tissue, which will be useful for discerning tumor margins. This research will advance the recent application of tissue print technology in bio-medicine by combining it with imaging and real time polymerase chain reaction (PCR) amplification and detection. A major objective in the design of this instrumentation is to develop the capacity to evaluate much larger areas of tissue. An approach to fulfilling this objective is the creation of a gasket that can seal individual wells of a nitrocellulose array. A gasket was created by laser cutting an assembly of molded silicone rubber and a double-sided tape (silicone-acrylic). Experiments showed when the gasket was adhered to a glass slide and subjected to the PCR, there was no leakage. FAST Slides, nitrocellulose slides provided by Grace Bio-Labs, are cut with a laser to generate the nitrocellulose arrays.en_US
dc.description.statementofresponsibilityby Helena Nien-Hwa Chia.en_US
dc.format.extent21 leavesen_US
dc.format.extent1543933 bytes
dc.format.extent1541996 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.language.isoengen_US
dc.publisherMassachusetts Institute of Technologyen_US
dc.rightsM.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.en_US
dc.rights.urihttp://dspace.mit.edu/handle/1721.1/7582
dc.subjectMechanical Engineering.en_US
dc.titleDevelopment of tissue printed nitrocellulose cards/arrays for real time PCR amplification and detectionen_US
dc.typeThesisen_US
dc.description.degreeS.B.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Dept. of Mechanical Engineering.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineering
dc.identifier.oclc57615839en_US


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