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dc.contributor.advisorRobert E. Cohen and Paula T. Hammond.en_US
dc.contributor.authorKim, Heejaeen_US
dc.contributor.otherMassachusetts Institute of Technology. Dept. of Materials Science and Engineering.en_US
dc.date.accessioned2006-07-31T15:16:47Z
dc.date.available2006-07-31T15:16:47Z
dc.date.copyright2005en_US
dc.date.issued2005en_US
dc.identifier.urihttp://hdl.handle.net/1721.1/33609
dc.descriptionThesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2005.en_US
dc.descriptionIncludes bibliographical references.en_US
dc.description.abstractTo fabricate living cell-based immunological sensors, we have examined two PEO-based biomaterials that can be patterned to generate cellular array templates: poly(allylamine)-g- poly(ethylene glycol) graft-copolymer and poly(ethylene glycol) dimethacrylate hydrogel. Poly(allylamine)-g-poly(ethylene glycol) polycation graft-copolymers were designed, synthesized, and characterized in order to combine bio-functionality with patternability on charged polyelectrolyte multilayer surfaces. Polymer-on-polymer stamping (POPS) techniques were used to create micron scale patterned regions on negatively charged multilayer surfaces via direct stamping of these graft copolymers. The long PEG side chains effectively resisted adsorption of antibodies or other proteins, and created a bio-inert area when patterned by POPS. On the other hand, desired proteins can be covalently attached to the graft copolymer by introducing proper coupling agents. Arrays of proteins were produced by either simple adsorption or coupling of proteins onto the graft copolymer patterned surfaces. The protein arrays were utilized as templates in fabricating cellular arrays of non-adherent B cells.en_US
dc.description.abstract(cont.) Poly(ethylene glycol) dimethacrylate hydrogel precursors were photopolymerized into 3D rmicrowell array templates via micromolding. After the floors of microwells were decorated with antibodies, hydrogel microwell array templates were employed in fabrication of cellular arrays. The topology of microwells facilitates the positioning of cells inside microwells and improves the binding stability of cells with protection from mechanical agitations. T cell arrays fabricated on hydrogel microwell array templates were tested as living cell- based immunological sensors. B cells were settled uniformly on T cell arrays to establish contacts between B and T cells over a large area. A level of T cell activation by target peptides were quantified using calcium sensitive fura dyes. A large set of individual T cell response data was acquired from a single T cell array and utilized to obtain average dose-response behavior of T cells. Average T cell responses were suitable as quantitative signal of a living cell-based immunological sensor. In addition, a potential application of T cell arrays in high throughput assay of individual T cell responses was investigated.en_US
dc.description.abstract(cont.) Analysis of calcium oscillation frequency of individual T cells revealed that there is no clear correlation of calcium oscillation frequency with target peptide dose in this experiment.en_US
dc.description.statementofresponsibilityby Heejae Kim.en_US
dc.format.extent133 leavesen_US
dc.format.extent8373299 bytes
dc.format.extent8378865 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.language.isoengen_US
dc.publisherMassachusetts Institute of Technologyen_US
dc.rightsM.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.en_US
dc.rights.urihttp://dspace.mit.edu/handle/1721.1/7582
dc.subjectMaterials Science and Engineering.en_US
dc.titleNew polymeric biomaterial interfaces for biosensor applicationsen_US
dc.typeThesisen_US
dc.description.degreePh.D.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Dept. of Materials Science and Engineering.en_US
dc.identifier.oclc64387362en_US


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