Nanoscale and microscale approaches for engineering the in vitro cellular microenvironment
Name
66294590-MIT.pdf
Description
Full printable version
Size
12.38 MB
Format
Adobe PDF
Checksum (MD5)
defd03ca6d75ce1364d3b21e06b705e6
Author(s)
Khademhosseini, Ali
Advisor(s)
Robert S. Langer.
Date Issued
2005
Publisher
Massachusetts Institute of Technology
Abstract
Micro- and nanofabrication approaches have dramatically changed our society through their use in microelectronics and telecommunication industries. These engineering tools are also useful for many biological applications ranging from drug delivery to DNA sequencing, since they can be used to fabricate small features at a low cost and in a reproducible manner. The goal of this thesis was to develop techniques based on the merger of novel materials and nano and microfabrication approaches to manipulate cell microenvironment in culture. To control cell migration and to restrict cell or colony size, cells and proteins were patterned by using molding or printing methods. Poly(ethylene glycol)-based molecules and polysaccharides were used to control cell-substrate interactions and to prevent cell adhesion on specific regions of a substrate. To control cell-cell contact, layer-by-layer deposition of ionic biopolymers (i.e. negatively charged hyaluronic acid and positively charged poly-L-lysine) was used to generate patterned co-cultures. In addition, to control cell-soluble factor interactions, microfluidic-based approaches were developed. To pattern cells and proteins within microchannels, a soft lithographic method was developed to pattern microchannel substrates using printing and molding approaches.
(cont.) To easily immobilize cells within channels, poly(ethylene glycol) microstructures were used to capture cells within low shear stress regions. These techniques also allowed for the fabrication of multiphenotype cell arrays. In addition, techniques were developed to control the interaction of cells within hydrogels by controlling the spatial properties of hydrogels.
Description
Thesis (Ph. D.)--Massachusetts Institute of Technology, Biological Engineering Division, 2005.
"June 2005."
Includes bibliographical references.
Subjects
Biological Engineering Division.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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