Size adjustable separation of biologically active molecules

Abstract

Separation of biologically active molecules (BAM's) is a problem for the pharmaceutical and biotechnology industries. Current technologies addressing this problem require too many techniques, toxic additives, and time to filter desired materials. As a result, a new technology is needed. The objective of this thesis is to contribute towards the development of a new method for separating biologically active molecules in the size range of 0.5 nanometers to 500 nanometers. A normally open diaphragm valve is proposed that can control a gap formed by two flat surfaces. For accurate control of gap height, the valve was designed to ensure that the flat surfaces remain parallel during operation . Modularity was also part of design considerations to address issues of eventual biocompatibility breakdown specifically protein adsorption. Control of the gap has been achieved to increments of 1.8 nanometers.