Isolation and characterization of bacterial polyhydroxybutyrate inclusions

Author(s)
Kshetry, Nina
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Massachusetts Institute of Technology. Dept. of Civil and Environmental Engineering.
Advisor
Cho Kyun Rha.
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Abstract
Polyhydroxybutyrate (PHB) is a carbon reserve found in some bacteria, and under nutrient limiting conditions accumulates intracellularly in the form of inclusion bodies. These inclusions contain proteins, and the PHB within the inclusions exists in an amorphous state. In this study a procedure to recover native PHB inclusions was developed, and the isolated inclusions were characterized using 13C NMR, western blotting, SDS-PAGE, atomic force microscopy, and fluorescence microscopy. A model for the structure of native PHB inclusions is proposed.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, 2006.
 
Includes bibliographical references (leaves 79-83).
 
Date issued
2006
URI
http://hdl.handle.net/1721.1/35690
Department
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
Publisher
Massachusetts Institute of Technology
Keywords
Civil and Environmental Engineering.
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