Isolation and characterization of bacterial polyhydroxybutyrate inclusions
Massachusetts Institute of Technology. Dept. of Civil and Environmental Engineering.
Cho Kyun Rha.
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Polyhydroxybutyrate (PHB) is a carbon reserve found in some bacteria, and under nutrient limiting conditions accumulates intracellularly in the form of inclusion bodies. These inclusions contain proteins, and the PHB within the inclusions exists in an amorphous state. In this study a procedure to recover native PHB inclusions was developed, and the isolated inclusions were characterized using 13C NMR, western blotting, SDS-PAGE, atomic force microscopy, and fluorescence microscopy. A model for the structure of native PHB inclusions is proposed.
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, 2006.Includes bibliographical references (leaves 79-83).
DepartmentMassachusetts Institute of Technology. Dept. of Civil and Environmental Engineering.
Massachusetts Institute of Technology
Civil and Environmental Engineering.