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Stabilization of Therapeutic Proteins
(2003-01)
We present results of molecular simulations, quantum mechanical calculations, and experimental data aimed towards the rational design of solvent formulations. In particular, we have found that the rate limitation of oxidation ...
Over Expression of the CMP-sialic Acid Transporter in Chinese Hamster Ovary Cells Leads to Increased Sialylation
(2005-01)
Most glyco-engineering approaches used to improve quality of recombinant glycoproteins involve the manipulation of glycosyltransferase and/or glycosidase expression. We investigated whether the over expression of nucleotide ...
CIRP Expression on Growth and Productivity of CHO Cells
(2006-01)
Mammalian cell culture is typically operated at the physiological temperature of 37°C. Low temperature cell culture at 30-33°C, in particular for CHO cells, increased the specific productivity of many recombinant proteins ...
Cell-line Engineering of Chinese Hamster Ovary Cells for Low-temperature Culture
(2005-01)
Developments in mammalian cell culture and recombinant technology has allowed for the production of recombinant proteins for use as human therapeutics. Mammalian cell culture is typically operated at the physiological ...
Reduced Temperature Production of Recombinant Proteins to Increase Productivity in Mammalian Cell Culture
(2004-01)
The production of recombinant proteins from an industrial perspective has one of its main goals is to increase the product concentration whether in batch, fed-batch or continuous perfusion bioreactor systems. However, a ...
Active Hypothermic Growth: A Novel Means For Increasing Total Interferon-γ Production by Chinese Hamster Ovary Cells
(2006-01)
When grown under hypothermic conditions, Chinese Hamster Ovary (CHO) cells become growth arrested in the G₀/G₁ phase of the cell cycle and also often exhibit increased recombinant protein production. In this study, we ...
Engineering Mammalian Cells for Improved Recombinant Protein Production
(2003-01)
The production of recombinant glycoproteins from mammalian cell cultures requires robust processes that can achieve high protein yield while ensuring the efficacy of these proteins as human therapeutics. We describe two ...
Cell-line Engineering for Low-temperature Growth
(2004-01)
In the chemical industry, the rates of reactions are usually enhanced by the use of high-temperature and high-pressure conditions. This chemical engineering approach is rarely applied in the biotechnology field. Firstly, ...
Enhancing Production of Recombinant Proteins from Mammalian Cells
(2003-01)
The bio-manufacturing of recombinant proteins from mammalian cell cultures requires robust processes that can maximize protein yield while ensuring the efficacy of these proteins as human therapeutics. Recognizing that the ...
The Effect of Culture Temperature on Recombinant IFN-γ Production and Quality
(2004-01)
The goal of this research project is to analyze the effect of culture temperature on the production and quality of IFN-γ produced and secreted by suspension culture CHO cells.The effect of low temperature on IFN-γ ...