Now showing items 1-5 of 5
Enhancing Production of Recombinant Proteins from Mammalian Cells
The bio-manufacturing of recombinant proteins from mammalian cell cultures requires robust processes that can maximize protein yield while ensuring the efficacy of these proteins as human therapeutics. Recognizing that the ...
Changes in the Overall Extent of Protein Glycosylation by Chinese Hamster Ovary Cells Over the Course of Batch Culture
Glycosylation of recombinant proteins can change during the culture of animal cells. Since lipid-linked oligosaccharides (LLOs) are the carbohydrate donors in N-linked glycosylation, their availability is postulated to ...
Glycosylation by Chinese Hamster Ovary Cells in Dolichol Phosphate-Supplemented Cultures
N-linked glycosylation often imparts important properties to protein therapeutics. An essential step in this intracellular process is the transfer of oligosaccharide from dolichol monophosphate (Dol-P) to a potential ...
Stabilization of Therapeutic Proteins
We present results of molecular simulations, quantum mechanical calculations, and experimental data aimed towards the rational design of solvent formulations. In particular, we have found that the rate limitation of oxidation ...
Engineering Mammalian Cells for Improved Recombinant Protein Production
The production of recombinant glycoproteins from mammalian cell cultures requires robust processes that can achieve high protein yield while ensuring the efficacy of these proteins as human therapeutics. We describe two ...