Volumetric reconstruction of tissue structure from two-dimensional microscopy images
Author(s)Cruz, Francisco (Francisco Ui)
Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.
Polina Golland and Richard T. Lee.
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Cell morphology of tissue is naturally three-dimensional. Most current methods for tissue analysis use two dimensional histological images of the tissue samples, restricting the analysis to 2D. Existing approaches do not provide essential three-dimensional information such as cell volume, shape and structural orientation of cells within the tissue. This thesis investigates a method to extract three dimensional data using two-dimensional microscopy. We demonstrate that three dimensional cell structure can be acquired using two dimensional fluorescence microscopy and two-photon microscopy and explore the application of the analysis to studies of cardiac tissue.
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, June 2006.Includes bibliographical references (leaf 41).
DepartmentMassachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology
Electrical Engineering and Computer Science.