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Changes in the Overall Extent of Protein Glycosylation by Chinese Hamster Ovary Cells Over the Course of Batch Culture

Author(s)
Yuk, Inn Huam Yvonne.; Wang, Daniel I.C.
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Abstract
Glycosylation of recombinant proteins can change during the culture of animal cells. Since lipid-linked oligosaccharides (LLOs) are the carbohydrate donors in N-linked glycosylation, their availability is postulated to influence the extent of glycosylation. To test this hypothesis, relative LLO and glycosylation levels in Chinese hamster ovary (CHO) cells were monitored over the course of batch culture for corresponding changes. Radiolabelling studies reveal that throughout the length of culture, intracellular LLO levels remained within a 2-fold range and overall CHO protein glycosylation varied by less than 30%. The implications of these results and hypotheses to explain the findings are discussed.
Date issued
2003-01
URI
http://hdl.handle.net/1721.1/3776
Series/Report no.
Molecular Engineering of Biological and Chemical Systems (MEBCS);
Keywords
G0G1 phase, lipid-linked oligosaccharide

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