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Extending imaging depth of multiphoton microscopy

Author(s)
Cha, Jae Won
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Massachusetts Institute of Technology. Dept. of Mechanical Engineering.
Advisor
Peter T.C. So.
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M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582
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Abstract
Two-photon excitation fluorescence microscopy has capability of deep tissue imaging with biological samples. However, because of the inhomogeneity of the refractive index in biological samples, the wavefront of the excitation light is often distorted. Due to the distortion of the wavefront, the point spread function at the focal point becomes broadened resulting in degraded resolution and lower signal. With an adaptive optics system, which consists of a wavefront camera and deformable mirror, the wavefront distortion can be measured and corrected. By correcting the distorted wavefront with adaptive optics, resolution and signal level can be preserved at greater imaging depth.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007.
 
Includes bibliographical references (leaves 45-46).
 
Date issued
2007
URI
http://hdl.handle.net/1721.1/42299
Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Publisher
Massachusetts Institute of Technology
Keywords
Mechanical Engineering.

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