Browsing Requests for Comments (RFCs) by Title

Now showing items 47-66 of 74

    • Measurement of bacterial promoter absolute activity and determination of dynamic performance in cell-free expression system by qPCR 

      Olchowik, Anna; Lower, Michal; Moreno, Cherry; Pankowski, Jaroslaw (2010-12-05)
      The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of bacterial promoters. The absolute activity can be experimentally measured in Moles of RNA per Moles of DNA in time, ...

    • The Measurement of rho‐independent Transcription Terminator Efficiency 

      He, Jiankui; Jing, Shui; Zhang, Mengshi; Liu, Xianggeng; Chen, Yao; e.a. (2012-10-11)
      The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of terminators in bacteria. Because we have characterized the performance of terminator in E. coli and used a simple ...

    • miMeasure – a standard for miRNA binding site characterization in mammalian cells 

      Adlung, Lorenz; Sabah, Jude Al; Bayer, Philipp; Berrens, Rebecca; Cristiano, Elena; e.a. (2010-12-05)
      This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, ...

    • The minimum information for a qualified BioBrick 

      Zhou, Mubing; Zhang, Junqiu; He, Jiankui (2012-10-11)
      Since the information of many existing BioBricks is incomplete, thus the usage of the BioBricks will be affected. It is necessary to standardize the minimum information required for a qualified BioBrick. Furthermore this ...

    • miTuner - a kit for microRNA based gene expression tuning in mammalian cells 

      Adlung, Lorenz; Sabah, Jude Al; Bayer, Philipp; Berrens, Rebecca; Cristiano, Elena; e.a. (2010-12-05)
      The purpose of this RFC is to introduce a modular expression tuning kit for use in mammalian cells. The kit enables the regulation of the gene expression of any gene of interest (GOI) based on synthetic microRNAs, endogenous ...

    • Naming of standards of physical composition of BioBrick parts 

      Rettberg, Randy; Shetty, Reshma (2009-04-17)
      This BioBricks Foundation Request for Comments (BBF RFC) proposes a naming convention for standards of physical composition of BioBrick parts in which assembly standards are named according to the BBF RFC that specifies ...

    • A New Standard to Connect BioBrick Parts for Precise Extraction of an Enzyme Digestion Product 

      Uekusa, Kousuke; Iguchi, Seiko (2010-12-05)
      This BioBricks Foundation Request for Comments (BBF RFC) introduces a new standard to connect BioBrick parts using BglI site.

    • Novel Normalization Standard using Fluorescence 

      Bukhari, Syed Habib Tahir; Akkineni, Ashwini Rahul; Ananth, Adithya; Gordeev, Victor; Grieb, Svea; e.a. (2010-12-05)
      The Biotec_Dresden Team 2010 developed an approach where two fluorescent proteins are simultaneously expressed. The fact that one reporter, in our case RFP, is constitutively expressed allows to monitor cell growth. Secondly, ...

    • Open licensing of BioBrick parts 

      Lower, Michal; Olchowik, Anna; Pankowski, Jaroslaw; Bazlekowa, Milena; Blaszkiewicz, Marta; e.a. (2010-12-05)
      This document provides recommendations for licensing of community-created biological parts, especially in BioBrick standard.

    • Open Sequence Initiative: a part submission standard to complement modern DNA assembly techniques 

      Hammerling, Michael J.; Gottel, Neil R.; Alnahas, Razan N.; Slater, Ben; Huang, Yunle; e.a. (2013-10-06)
      The discipline of synthetic biology emphasizes the application of engineering principles such as standardization, abstraction, modularity, and rational design to complex biological systems. The archetypical example of such ...

    • PCR - Ligation Assembly Standard for BioBrick Parts 

      He, Tony PeiYuan; Campbell, Alexander; Zhou, Stefanie Yifan (2011-12-15)
      This Request for Comments (RFC) describes a novel method for the assembly of standard BioBrick parts. This assembly method for BioBrick parts is an improvement upon the conventional methods of BioBrick part assembly. This ...

    • Promoter and Coding Sequence Considerations for Caenorhabditis elegans and Other Eukaryotes 

      Palmer, Chris; Halliday, Geoff (2010-11-07)
      Our primary aim is to provide a standard format for protein-coding BioBricks intended for eukaryotes, especially in the nematode worm Caenorhabditis elegans, so that future teams will be able to correctly take advantage ...

    • Proposed Conceptual Guidelines for the Design of a BioBrick Graphical Language & an Example 

      Fernandez, Eric (2009-03-12)
      As we update the current BioBrick symbols we have an opportunity to look at the end users of a BioBrick Graphical language and develop design guidelines so that the new symbols best suit their needs. This document explores ...

    • Provisional BioBrick Language (PoBoL) 

      Galdzicki, Michal; Chandran, Deepak; Nielsen, Alec; Morrison, Jason; Cowell, Mackenzie; e.a. (2009-05-15)
      This BioBricks Foundation Request for Comments (BBF RFC) describes a semantic markup language for publishing and sharing information about BioBricks on the World Wide Web. This BBF RFC includes the recommendation for the ...

    • Quantitative measurement of mammalian cell invasion by bacteria using flow cytometry 

      Lower, Michal; Olchowik, Anna (2010-10-31)
      Purpose of this RFC is to provide method of measurement of bacterial invasion into mammalian cells. Also this RFC defines Percent of INvasion (PIN) unit.

    • RA-PCR, a method for the generation of randomized promoter libraries 

      Velten, Lars; Haas, Simon; Rademacher, Anne; Meyer, Hannah; Eils, Roland (2009-10-21)
      The purpose of this RFC is to provide instructions for the synthesis of promoters in mammalian cells that are active at a desired cellular condition (where a cellular condition is specified by the activity of a set of ...

    • Recombination Based Part Assembly 

      Deming, Laura; Slusarczyk, Adrian; Jiao, Yunxin; iGEM, 2010 MIT (2010-12-05)
      Here we propose a new recombination-based assembly standard, optimized to allow for efficient cloning of mammalian vectors.

    • A RESTful API for Supporting Automated BioBrick Model Assembly 

      Steyn, J S; Boyd, R M; Essa, Y; Hall, P; Koh, A; e.a. (2010-12-05)
      Constructing simulatable models for BioBricks by hand is a complex and time-consuming task. The time taken could be reduced by using Computer Aided Design (CAD) tools to aid in designing models, but these tools need to be ...

    • Reusable rapid assembly of genetic parts for Neurospora crassa 

      Odsen, Raymond; Baretta, Kayla; Zakrewski, Helena; Simpson, Michael; Guo, Yuan; e.a. (2011-10-17)
      This BBF RFC provides a method in which standardized parts can be easily created for Neurospora crassa.

    • Standard Biological Part Automatic Modeling Database Language (MoDeL) 

      Wang, Zhen; Liao, Chen; Jiang, Hao; Yao, Xiaomo; Jiang, Kun (2010-11-09)
      This BioBricks Foundation Request for Comments (BBF RFC) describes the Standard Biological Part Automatic Modeling Database Language (MoDeL). MoDeL provides a language and syntax standard for automatic modeling databases ...