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dc.contributor.advisorThomas F. Knight, Jr.en_US
dc.contributor.authorHuang, Haiyaoen_US
dc.contributor.otherMassachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.en_US
dc.date.accessioned2009-06-30T16:54:37Z
dc.date.available2009-06-30T16:54:37Z
dc.date.copyright2007en_US
dc.date.issued2007en_US
dc.identifier.urihttp://hdl.handle.net/1721.1/45981
dc.descriptionThesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2007.en_US
dc.descriptionIncludes bibliographical references (p. 103-104).en_US
dc.description.abstractDesign and characterization of artificial transcriptional terminators. Ten new terminators were designed based on previous research of terminator structure and termination efficiency. The terminators were built by PCR extension, ligated into a BioBrick plasmid backbone, and transformed into TOP10 cells. Characterization devices were built to test the terminators. Input and output of the terminator were measured by expression of RFP and GFP. Charaterization devices were then placed into the E. coli strain CW2553/pJAT18, which hijacks the arabinose transport system to provide controlled input to the terminator. Of the ten terminators designed and tested, BBa_B1002, BBa_B1004, BBa_B1006 and BBa_B1010 proved to be strong terminators with termination efficiencies above 90%. These terminators may be obtained from the Registry of Standarized Parts at parts.mit.edu.en_US
dc.description.statementofresponsibilityby Haiyao Huang.en_US
dc.format.extent104 p.en_US
dc.language.isoengen_US
dc.publisherMassachusetts Institute of Technologyen_US
dc.rightsM.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.en_US
dc.rights.urihttp://dspace.mit.edu/handle/1721.1/7582en_US
dc.subjectElectrical Engineering and Computer Science.en_US
dc.titleDesign and characterization of artificial transcriptional terminatorsen_US
dc.typeThesisen_US
dc.description.degreeM.Eng.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
dc.identifier.oclc334966967en_US


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