Development of an integrated capillary valve-based preconcentrator and surface-based immunoassay
Author(s)
Liu, Vincent Hok![Thumbnail](/bitstream/handle/1721.1/47815/429466732-MIT.pdf.jpg?sequence=5&isAllowed=y)
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Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.
Advisor
Jongyoon Han.
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A new generation of integrated preconcentrator and immunoassay was developed. A novel, self-aligned method for patterning Nafion resin was developed and applied to create a preconcentrator. In a parallel effort, a surface-based immunoassay was developed and optimized for use with the preconcentrator. Experiments were carried out with R-phycoerythrin (R-PE) to characterize the performance of the integrated preconcentrator and immunoassay. The immunoassay originally has a detection limit of Ing/mL of R-PE in 1xPBS. When coupled to the preconcentrator, this limit has been improved to 0.Olng/mL, a roughly 100x improvement. Discussions of the differences between this and previous generations of concentrators are also presented in this thesis.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2009. Includes bibliographical references (leaves 60-61).
Date issued
2009Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer SciencePublisher
Massachusetts Institute of Technology
Keywords
Electrical Engineering and Computer Science.