Development of an integrated capillary valve-based preconcentrator and surface-based immunoassay
Author(s)Liu, Vincent Hok
Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.
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A new generation of integrated preconcentrator and immunoassay was developed. A novel, self-aligned method for patterning Nafion resin was developed and applied to create a preconcentrator. In a parallel effort, a surface-based immunoassay was developed and optimized for use with the preconcentrator. Experiments were carried out with R-phycoerythrin (R-PE) to characterize the performance of the integrated preconcentrator and immunoassay. The immunoassay originally has a detection limit of Ing/mL of R-PE in 1xPBS. When coupled to the preconcentrator, this limit has been improved to 0.Olng/mL, a roughly 100x improvement. Discussions of the differences between this and previous generations of concentrators are also presented in this thesis.
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2009.Includes bibliographical references (leaves 60-61).
DepartmentMassachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.
Massachusetts Institute of Technology
Electrical Engineering and Computer Science.